摘要
本试验旨在研究枯草芽孢杆菌对围产期母猪粪便微生物及其代谢产物的影响。试验选用2~4胎次、预产期相近、妊娠第85天的健康大白猪40头,随机分为2个组,每组20头猪。对照组饲喂基础饲粮,试验组在基础饲粮中添加250 g/t的枯草芽孢杆菌制剂。从母猪妊娠第85天开始饲喂至产后第21天结束。分别于母猪妊娠第100和112天以及产后第7、14和21天每组采集8头母猪的新鲜粪便样品,测定其粪便中微生物数量、短链脂肪酸(SCFA)和生物胺含量。结果表明:与对照组相比,1)饲粮中添加枯草芽孢杆菌显著增加产后第7天母猪粪便中乳酸杆菌数量、乳酸杆菌/大肠杆菌和产后第21天粪便中厚壁菌数量(P<0.05)。2)饲粮中添加枯草芽孢杆菌显著增加妊娠第100天母猪粪便中乙酸、异丁酸、支链脂肪酸和总SCFA含量(P<0.05),产后第7天粪便中戊酸、直链脂肪酸、异丁酸、异戊酸、支链脂肪酸和总SCFA含量(P<0.05),产后第21天粪便中丙酸、丁酸和直链脂肪酸含量(P<0.05);显著降低产后第14天粪便中异戊酸含量(P<0.05)。3)饲粮中添加枯草芽孢杆菌显著增加妊娠第100天母猪粪便中尸胺含量(P<0.05),妊娠第112天粪便中亚精胺和精胺含量(P<0.05),产后第14天粪便中尸胺、酪胺、亚精胺和精胺含量(P<0.05)。综上所述,围产期母猪饲粮中添加枯草芽孢杆菌可增加肠道中有益菌数量、部分生物胺和SCFA含量,对改善母猪的肠道健康和营养状况具有有益作用。
This study was conducted to determine the effects of Bacillus subtilis on fecal microbes and their metabolites of peripartum sows.Forty healthy Large White sows at close to day 85 of pregnancy during 2 to 4 parities were used and randomly allocated to 2 groups with 20 pigs per group.The sows in the control group were fed a basal diet,and the others in the experimental group were fed the basal diet supplemented with 250 g/t Bacillus subtilis preparation.The trial period was from day 85 of pregnancy to day 21 of postpartum.Eight sows per group were randomly selected and the fresh feces were collected at days 100 and 112 of pregnancy and days 7,14 and 21 of postpartum,respectively,and the microbial population and the contents of short chain fatty acids(SCFA)and bioamines of feces were analyzed.The results showed as follows:compared with the control group,1)dietary Bacillus subtilis significantly increased the fecal Lactobacillus population and the ratio of Lactobacillus to Escherichia coli of sows at day 7 of postpartum and fecal Firmicutes population at day 21 of postpartum(P<0.05).2)Dietary Bacillus subtilis significantly increased the contents of fecal acetate,isobutyrate,branched chain fatty acid and total SCFA of sows at day 100 of pregnancy(P<0.05),the contents of fecal valerate,straight-chain fatty acids,isobutyrate,isovalerate,branched chain fatty acid and total SCFA at day 7 of postpartum(P<0.05),and the contents of fecal propionate,butyrate and straight-chain fatty acids at day 21 of postpartum(P<0.05).But dietary Bacillus subtilis significantly decreased the fecal isovalerate content of sows at day 14 of postpartum(P<0.05).3)Dietary Bacillus subtilis significantly increased the fecal cadaverine content of sows at day 100 of pregnancy(P<0.05),the contents of fecal spermidine and spermine at day 112 of pregnancy(P<0.05),and the contents of fecal cadaverine,tyramine,spermidine and spermine at day 14 of postpartum(P<0.05).Collectively,dietary Bacillus subtilis can increase the intestinal beneficial microbes population and contents of several bioamines and SCFA of perinatal sows,which plays important roles in improving intestinal health and nutritional status of sows.
作者
韩丽
解培峰
赵越
高乾坤
章文明
张涛
王占彬
印遇龙
孔祥峰
HAN Li;XIE Peifeng;ZHAO Yue;GAO Qiankun;ZHANG Wenming;ZHANG Tao;WANG Zhanbin;YIN Yulong;KONG Xiangfeng(Key Laboratory of Agro-Ecological Processes in Subtropical Region,Laboratory of Animal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471003,China;Evonik Degussa(China)Co.,Ltd.,Beijing 100600,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2018年第3期1116-1124,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家重点研发计划课题(2017YFD0500503)；赢创德固赛(中国)投资有限公司横向课题
作者简介
韩丽（1992—），女，河南周口人，硕士研究生，从事动物营养与饲料科学研究。E-mail:1404082917@qq.com;通信作者:孔祥峰，研究员，博士生导师，E-mail:nnkxf@isa.ac.cn
