摘要
目的分析环状RNA(circular RNA,circRNA)在口腔鳞状细胞癌组织及癌旁组织中表达谱的差异及临床意义。方法利用高通量芯片技术检测3例口腔鳞状细胞癌患者癌组织和配对癌旁组织中的差异表达circRNA,采用实时定量PCR(quantitative real-time PCR,RT-qPCR)方法验证所筛选的部分circRNA分子在45对口腔鳞状细胞癌组织与癌旁组织中的表达情况。分析上调明显的circRNA表达水平与口腔鳞状细胞癌临床病理特征的关系,采用Arraystar公司circRNA靶基因分析软件,预测可能与上调明显的circRNA相互作用的靶miRNA分子。结果口腔鳞状细胞癌患者癌组织与癌旁组织样本间差异表达的circRNA共155个,其中上调45个,下调110个。所挑选的在口腔鳞状细胞癌组织上调或下调差异表达最显著的3条circRNA中RT-qPCR验证结果显示,与癌旁组织相比,口腔鳞癌癌组织中hsa_circ_0001874、hsa_circ_0001971、has_circ_0067934表达上调,hsa_circ_0000520、hsa_circ_0023944、hsa_circ_0000140表达下调,与芯片检测结果一致。在上述芯片筛选的口腔鳞状细胞癌组织特征circRNA表达谱中,hsa_circ_0001874上调表达最明显,hsa_circ_0001874的表达在不同临床分期及细胞分化程度存在显著差异;circRNA靶基因分析软件预测结果提示,miR-103a-3p、miR-107、miR-593-5p、miR-661和miR-662可能是hsa_circ_0001874的潜在靶基因。结论多种circRNA在口腔鳞状细胞癌组织中呈异常表达,这些表达差异的circRNA及其潜在的靶基因可能与口腔鳞状细胞癌的发生、发展密切相关。
Objective To analyze circular RNA(circRNA)expression profiles in oral squamous cell carcinoma(OSCC)and its clinical significance.Methods The expression of circRNA was detected with circRNA microarray assay in three samples of OSCC tumor and matched adjacent tissues.Quantitative real-time PCR(RT-qPCR)was used to verify the expression of circRNA in 45 pair OSCC tissues and normal adjacent tissues.The relationship between the expression of circRNA and the clinicopathological characteristics of OSCC was analyzed.circRNAs/miRNAs interaction were predicted using Arraystar’s home?made miRNA target prediction software.Results 155 circRNAs were differentially expressed between the OSCC tissues and matched adjacent tissues,of which 45 circRNAs were up-regulated and 110 circRNAs were downregulated in OSCC tissues(fold changes≥1.5 and P<0.05).In the selected three circRNAs that were most significantly upregulated or downregulated in OSCC,the RT-qPCR results showed that hsa_circ_0001874,hsa_circ_0001971 and has_circ_0067934 were increased,while hsa_circ_0000520,hsa_circ_0023944 and hsa_circ_0000140 were decreased in OSCC tissues versus normal adjacent tissues(P<0.001).The results were generally consistent with the microarray data.Among the circRNA expression profiles in OSCC,the upregulation of hsa_circ_0001874 was the highest and the expression of hsa_circ_0001874 was significantly correlated with TNM stage and tumor grade.The result of Arraystar’s homemade miRNA target prediction software indicated that miR-103a-3p,miR-107,miR-593-5p,miR-661 and miR-662 may be potential target genes of hsa_circ_0001874.Conclusion The differentially expressed circRNAs in OSCC tissues and normal adjacent tissues were identified,and these dysregulated cir?cRNAs and their potential target gents may play important roles in the development of OSCC.
作者
赵思语
欧阳少波
王军
黄自坤
罗清
廖岚
ZHAO Siyu;OUYANG Shaobo;WANG Jun;HUANG Zikun;LUO Qing;LIAO Lan(Department of Oral Prosthodontics,Affiliated Stomatological Hospital of Nanchang University,the Key Laboratory of Oral Biomedicine in Jiangxi Provinice,Nanchang 330006,China;Department of Oral and Maxillofacial Surgery,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of Clinical Laboratory,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China)
出处
《口腔疾病防治》
2018年第2期83-89,共7页
Journal of Prevention and Treatment for Stomatological Diseases
基金
国家自然科学基金项目(81660444)
江西省自然科学基金项目(20171BAB205050)
江西省重点研发计划项目(20161BBG70149)
江西省卫生和计生委科技项目(20175296)
关键词
环状RNA
口腔
鳞状细胞
癌
癌旁组织
基因芯片
RT-QPCR
靶基因
Circular RNA
Oral
Squamous cell
Carcinoma
Matched adjacent tissues
Microarray
Quantitative real-time PCR
Target genes
作者简介
赵思语,医师,硕士研究生在读,Email:531909402@qq.com;【通信作者】廖岚,主任医师,博士生导师,Email:liaolan5106@163.com
