摘要
以在大田已结商品苦瓜植株的顶芽和侧芽作外植体为主,进行组培快繁研究,针对不同株龄的外植体,比较了不同的激素类型及配比组合对诱导不定芽形成、芽增殖以及不定根形成的影响,建立了大田苦瓜的离体再生体系。结果表明:不同株龄的外植体在适宜的培养基中均能很好地被诱导,但株龄越长的材料诱导不定芽所需时间越长。苦瓜不定芽诱导的最适培养基为MS+6-BA 0.3 mg/L+NAA 0.3 mg/L,不定芽增殖最适培养基为MS+6-BA 0.1 mg/L,不定根形成最适培养基为MS+IBA 0.1 mg/L。在相同栽培条件下,苦瓜组培苗长势比实生苗弱,但是其果实性状与实生苗无异,且具有提早成熟、整齐度高等优点,因此在苦瓜生产上,应改进栽培条件,逐步推行组培苗作为生产用苗。
The in vitro terminal bud and axillary of bitter gourd(Momordica charantia Linn.)were used for tissue culture to establish a high-efficiency regeneration system,and the effects of different hormone combinations and culture medium on the adventitious bud induction,multiplication and radication for the in vitro of different ages were studied.The results showed that the in vitro of different ages could be induced well in appropriate induction media,with longer time needed for the induction of bud for older in vitro.It was found that the most appropriate induction medium for the adventitious buds induction and multiplication were MS+6-BA0.3mg/L+NAA0.3mg/L and MS+6-BA0.1mg/L;the appropriate radication media was MS+IBA0.1mg/L.Under the same culture condition,the seedlings from tissue culture had smaller plant growth but comparable fruit characteristics,earlier ripening time and higher uniformity,compared to the seed-grown seedlings,suggesting the importance of improved cultivation practice with application of tissue culture seedlings in bitter gourd field production.
作者
吴蓓
戴修纯
郑岩松
张木清
李光光
陈胜文
刘绍钦
WU Bei;DAI Xiu-chun;ZHENG Yan-song;ZHANG Mu-qing;LI Guang-guang;CHEN Sheng-wen;LIU Shao-qin(Guangzhou Academy of Agricultural Sciences,Guangzhou 510335,China)
出处
《广东农业科学》
CAS
2017年第12期27-32,共6页
Guangdong Agricultural Sciences
基金
广州市科技计划项目(201508020066)
广东省科技计划项目(2014A020208005)
关键词
苦瓜
大田栽培
离体
组织培养
组培苗
实生苗
bitter gourd( Momordica charantia Linn.)
field cultivation
in vitro
rapid propagation
tissue seedling
seed-grown seedling
作者简介
吴蓓(1979-),女,博士,高级农艺师,E-mail:wubei7979@qq.com
