摘要
在pH 7.0的磷酸盐缓冲液中,葡萄糖氧化酶(GOx)催化葡萄糖与氧反应生成H_2O_2,在H_2SO_4介质中H_2O_2与I^-发生氧化还原反应生成I_2,过量的I^-与I_2生成I_3^-,I_3^-使阳离子荧光剂罗丹明6G位于553 nm波长处的荧光发生猝灭,猝灭程度与葡萄糖浓度相关,据此建立检测葡萄糖的新方法。方法线性范围为2.0×10^(-7)~1.4×10^(-5)mol/L,检出限为6.77×10^(-8)mol/L。将该方法用于血糖样品测定,加标回收率为85.0%~102.5%,操作简便、结果准确可靠。
In phosphate buffer solution(pH7.0),glucose oxidase(GOx)can catalyze the reaction of glucose with oxygen generate hydrogen peroxide,then hydrogen peroxide reaction with excess I-in dilute sulfuric acid solution to form I-3.The I-3 combines with Rhodamine 6G(Rh6G)to form Rh6G-I3 associated particles which result in fluorescence quenching at 553 nm.Based on the significant quenching effect of glucose on the fluorescence of Rh6G,a new method with high sensitivity and good selectivity for the determination of glucose was established.Under the optimal conditions,the concentration of glucose in the range of 2.0×10-7~1.4×10-5 mol/L is linear with their fluorescence quenching at 553 nm,with detection limits of 6.77×10-8 mol/L glucose.This assay was applied to the determination of glucose in the serum samples with satisfactory results,the recoveries obtained were 85.0%~102.5%.
作者
何顺珍
李菲菲
陆一松
林茜
徐小娜
杨胜园
HE Shun-zhen;LI Fei-fei;LU Yi-song;LIN Xi;XU Xiao-na;YANG Sheng-yuan(College of Public Health,University of South China,Hengyang 421001,China;Affiliated Nanhua Hospital,University of South China,Hengyang 421001,China)
出处
《应用化工》
CAS
CSCD
北大核心
2018年第3期611-613,共3页
Applied Chemical Industry
基金
国家自然基金青年基金项目支持(11205085)
湖南省教育厅优秀青年项目资助(15B202)
作者简介
何顺珍(1990-),女,贵州遵义人,南华大学在读硕士,师从杨胜园教授,主要从事化学生物物质分析检测研究。电话:0734-8281393,E-mail:1039497963@qq.com;通讯联系人:杨胜园(1975-),女(土家族),湖南吉首人,教授,博士,硕士研究生导师,主要从事化学生物物质分析检测研究。电话:13873443231,E-mail:yangshyhy@126.com
