摘要
猪嵴病毒(porcine kobuvirus,PKV)是近年来在健康猪和腹泻猪粪便中新检测到的小核糖核酸病毒科嵴病毒属成员,可能引起猪的腹泻,对养猪业造成重大经济损失。研究发现,PKV广泛分布在猪中,在腹泻和临床健康猪中均已被检测到,阳性率从3.9%~100.0%各不相同。一个典型的PKV病毒粒子直径为30nm,基因组全长为8 120bp,包括1个含2 488个氨基酸的开放性阅读框(ORF);PKV是典型的小RNA病毒科的基因组结构:1个5′非编码区,1个L蛋白,结构蛋白P1(VP0、VP3和VP1),非结构蛋白P2(2A、2B和2C)和P3(3A、3B、3C和3D),1个3′非编码区和1个Poly(A)尾巴。PKV的2B编码区存在30个氨基酸的缺失,VP1蛋白是小RNA病毒科变异最频繁的结构蛋白,其含有主要的抗原表位,可促进机体产生中和抗体。检测PKV的方法有反转录聚合酶链式反应(RT-PCR)、TaqMan探针实时荧光定量RT-PCR和逆转录环介导扩增(RT-LAMP)方法。作者对PKV的分类学、流行概况、基因组结构、遗传特性及检测技术等研究现状做一简要概述,以期为进一步研究及了解PKV提供参考。
orcine kobuvirus(PKV)is a member of the newly detected in the genus Kobuvirus,family Picornaviridae in healthy pigs and diarrhea pigs.It may cause diarrhea of pigs and cause significant economic losses to pig industry.At present,the studies find that,PKV is widely distributed in pigs and has been detected in diarrhea and clinically healthy pigs,and the positive rate of the virus varies from3.9%to100.0%.A typical porcine kobuvirus virion is30nm in diameter,and its genome is8120bp in length and contains a single open reading frame(ORF)encoding a protein of2488amino acids.The genome organization is typical for picornaviruses:A5′UTR,a leader(L)protein,structural proteins P1(VP0,VP3and VP1),non-structural proteins P2(2A,2B and2C)and P3(3A,3B,3C and3D),a3′UTR and a Poly(A)tail.There are30amino acid deletions in the2B coding region of the porcine kobuvirus,VP1protein is the most frequent variant of the small RNA virus,structural protein,which contains the main epitope,can promote the body to produce neutralizing antibodies.At present,the methods of detecting PKV are reverse transcription polymerase chain reaction(RT-PCR),TaqMan probe Real-time RT-PCR and reverse transcription-loop mediated amplification(RT-LAMP).In this paper,a brief overview of the taxonomy,epidemiological profile,genome structure,genetic characteristics and detection techniques of PKV was made,so as to provide references for the further study and understand PKV.
作者
黄晓星
王仙
金文杰
HUANG Xiao-xing;WANG Xian;JIN Wen-jie(Jiangsu Provincial Co-innovation Centrr f r r Prevention and Control o f Important Animal Infectious Disease and Zoonosss, Jiangsu Provincial Key Laboratory o f Animal Preventive Medicine, College o f Veterinary Medicine, Yangzhou University, Yangzhou 225009, China)
出处
《中国畜牧兽医》
CAS
北大核心
2017年第12期3466-3472,共7页
China Animal Husbandry & Veterinary Medicine
基金
江苏省“青蓝工程”
江苏高校优势学科建设工程资助项目(PAPD)
江苏省农业三新工程(SXGC[2015]077)
常州市科技支撑项目(CE20152006)
关键词
猪嵴病毒(PKV)
流行病学
基因组结构
遗传特性
检测技术
porcine kobuvirus (PKV)
epidemiology
genome structure
genetic characteristics
detection technique
作者简介
黄晓星（1993-），女，四川南部人，硕士生，研究方向：猪腹泻性疾病，E-mail：1016472346@qq.com;通信作者： 金文杰(1973-)，男，江苏武进人，博士，副教授，研究方向：兽医微生物学与免疫学，E-mail: wenjiejin1@163.com
