摘要
目的考察两面针中木脂素化合物结晶-8(结晶-8)是否在中枢发挥镇痛作用,并探讨结晶-8与受体的关系。方法将32只SD大鼠随机分为0.9%氯化钠溶液组、罗通定组(2 mg·kg^(-1))、结晶-8大剂量组(2 mg·kg^(-1))、结晶-8组小剂量组(1 mg·kg^(-1)),侧脑室注射给药,光热刺激法观察大鼠痛阀变化。将SD大鼠或小鼠,随机分为0.9%氯化钠溶液组,受体工具药组(纳洛酮、利血平、氟哌啶醇、东莨菪碱),结晶-8组、受体工具药+结晶-8组,给药后15,30,45,60,90,120 min通过光热刺激法测定痛阀,观察结晶-8镇痛作用与神经递质的关系。结果与0.9%氯化钠溶液组比较,结晶-8大、小剂量组大鼠痛阀值提高(P<0.01)。与结晶-8组比较,纳络酮+结晶-8组、氟哌啶醇+结晶-8组、东莨菪碱+结晶-8组痛阈值差异无统计学意义(P>0.05),利血平+结晶-8组痛阀值降低,差异有统计学意义(P<0.01),结晶-8镇痛作用被利血平拮抗。结论结晶-8镇痛机制可能在中枢,镇痛机制与中枢递质有关,但与阻断中枢DA受体无关,阿片受体、M胆碱受体未参与镇痛机制。
ObjectiveTo study whether crystal8exerts analgesic effect on the central nervous system,and to observe the relationship between crystal8and receptors.MethodsA total of32SD rats were randomly divided into the0.9%sodium chloride solution group,positive control group(rotundine2mg·kg-1),high dose of crystal8group(2mg·kg-1)and low dose of crystal8group(1mg·kg-1).The changes of pain threshold were measured by the thermal stimulation test following intracerebroventricular(ICV)injection.The SD rats or mice were randomly divided into the0.9%sodium chloride solution group,receptor tool medicine group(including naloxone,reserpine,haloperidol,scopolamine),crystal8group,receptor tool medicine plus crystal8group.The pain threshold was detected by the thermal stimulation test at15,30,45,60,90,120min after dosing,then the influence of analgesic effect of crystal8on the neurotransmitter was observed.ResultsCompared with the0.9%sodium chloride solution group,the pain threshold of rats was improved after taking the crystal8by intracerebroventricular(ICV)injection(P<0.01).Compared with the crystal8group,the naloxone plus crystal8group,the haloperidol plus crystal8group,the scopolamine plus crystal8group couldn’t increase the pain threshold,there was no significant difference among these groups(P>0.05).However,Reserpine plus crystal8group could significantly decrease the pain threshold in rats compared with the crystal8group(P<0.01).The analgesic effect of crystal8was interfered by reserpine.ConclusionThe analgesic effect of crystal8may be involved in the central mechanism,which relates to monoamine neurotransmitters,but has nothing to do with the opioid receptor,M receptor,and the inhibition of central DA receptor.
作者
王希斌
黄慧学
刘华钢
WANG Xibin;HUANG Huixue;LIU Huagang(Department of Pharmacy,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;College of Pharmacy,Guangxi Traditional Chinese Medical University,Nanning 530022,China;College of Pharmacy, Guangxi Medical University,Nanning 530021,China)
出处
《医药导报》
CAS
2017年第11期1240-1243,共4页
Herald of Medicine
基金
高等学校博士学科点专项科研基金资助项目(20050598003)
关键词
两面针
木脂素
化合物结晶-8
镇痛机制
Zanthoxylum nitidum (Roxb)
Lignans
Compound crystal-8
Analgesia mechanism
作者简介
王希斌(1977-),男,广西玉林人,主管药师,硕士,研究方向:中药药理、医院药学。电话:0771-5356154,E-mail:764822453@qq.com。;通信作者刘华钢(1956-),女,教授,博士生导师,研究方向:中药新剂型、新制剂及中药药理机制。E-mail:46669073@qq.com。
