摘要
以正常生长和高温干旱复合胁迫下甘蓝型油菜中双10号的茎和根为材料,采用组织化学、生物化学、气相色谱-质谱联用(GC-MS)分析技术,研究了木质部结构和木质素成分的胁迫应答规律及其在茎和根中的差别。冰冻切片组织化学染色显示,与正常生长的网室植株(正常植株)相比,高温干旱下生长的温室植株(胁迫植株)的茎和根中木质部均显著加厚,染色更深;与此对应,溴乙酰法测定的茎木质素总量比对照增加31.64%。此外,胁迫茎中的导管孔径明显变小,但根中的导管孔径和导管数量均明显增加。硫代酸解法测定木质素单体表明,胁迫茎中被解离出的木质素单体总量比对照降低40.08%,说明有更高的缩合键比例;S/G值(1.82)比对照(1.29)大大增高,说明S型木质素比例增加而G型木质素比例下降。油菜茎与根木质化性状比较显示,根木质素比茎木质素含有更高比例的缩合键,茎中S型木质素占主体(S/G=1.29),而根中G型木质素占主体(S/G=0.49)且H型木质素含量(7.43%)比茎中(0.83%)高近10倍。H型和G型木质素单体的苯环甲基化程度比S型低,单体间更容易形成缩合键,根中高比例H型和G型木质素单体可能是导致其具有高比例缩合键的主要原因。
Using histochemical,biochemical,and gas chromatography-mass spectrometry(GC-MS)technologies,the responsive trends of xylem structure and lignin components in the stem and the root of rapeseed(Brassica napus)cultivar Zhongshuang10under heat and drought stresses were investigated as compared with normal-growth plants.Histochemical staining of the frozen sections showed that,compared with the cage-grown plants(normal plants),the plants grown in greenhouse(stressed plants)had a thicker xylem part in both stem and root,with deeper staining color.Correspondingly,the total lignin content in the stem of stressed plants tested by acetyl bromide method increased by31.64%compared with that of the normal plants.Besides,heat and drought compound stress reduced vessel inner diameter in the stem,while increased both vessel number and vessel inner diameter in the root.Stem total lignin monomer yield prepared by thioacidolysis of stressed plants was40.08%lower than that of the normal plants,indicating higher condensed bonds in stressed plants.Meanwhile,the S/G ratio(1.82)was significantly higher than that of the normal plants(1.29),indicating increased proportion of S units and decreased proportion of G units.Furthermore,this study also revealed the difference of lignification traits between the stem and the root.The stem had lower condensed bonds and major proportion of S units(S/G=1.29),while the root had more condensed bonds and major proportion of G units(S/G=0.49).Moreover,the H unit percentage in the root(7.43%)was almost10folds as that in the stem(0.83%).Since H and G units have lower methylation levels and are easier to form condensed bonds,the high proportions of H and G units might be the main reason for the high proportion of condensed bonds in root lignin structure
作者
尹能文
李加纳
刘雪
练剑平
付春
李威
蒋佳怡
薛雨飞
王君
柴友荣
YIN Neng-Wen;LI Jia-Na;LIU Xue;LIAN Jian-Ping;FU Chun;LI Wei;JIANG Jia-Yi;XUE Yu-Fei;WANG Jun;CHAI You-Rong(Chongqing Rapeseed Engineering Research Center / Chongqing Key Laboratory of Crop Quality Improvement / Engineering Research Center of South Upland Agriculture of Ministry of Education / College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China)
出处
《作物学报》
CAS
CSCD
北大核心
2017年第11期1689-1695,共7页
Acta Agronomica Sinica
基金
教育部高等学校博士学科点专项科研基金项目(20130182110006)
国家重点基础研究发展计划(973计划)项目(2015CB 150201)
国家自然科学基金项目(31171177
31371238)
国家重点研发计划试点专项(2016YFD0100506)
重庆市基础科学与前沿技术研究专项重点项目(cstc2015jcyj BX0143)资助~~
关键词
油菜
茎
根
木质素
高温干旱胁迫
Rapeseed (Brassica napus)
Stem
Root
Lignin
Heat and drought stresses
作者简介
尹能文, E-mail: nwyin80@126.com;通讯作者(Corresponding author): 柴友荣, E-mail: chaiyourong@163.com
