摘要
以青藏高原极端盐湖为研究对象,采用16S r RNA基因(V3~V4区)高通量测序方法分析盐湖细菌的群落结构差异和超盐环境因子的制约相关性.结果显示,超盐环境样本中细菌的群落结构组成相对稳定,进化趋同,且复杂度和多样性指数显著低于非超盐环境.青藏高原超盐环境中细菌的优势类群是厚壁菌门Firmicutes(74.04%~81.04%)、次为变形菌门Proteobacteria(15.51%~20.06%)、拟杆菌门Bacteroidetes(2.68%~4.84%)和放线菌门Actinobacteria(0.71%~2.45%).比较分析其它类型超盐环境,青藏高原超盐环境具有高丰度的特色属群芽孢杆菌属Bacillus(50.63%~58.35%)、乳球杆菌属Lactococcus(9.28%~11.81%)和海洋芽孢杆菌属Oceanobacillus(8.41%~10.52%).基于典范对应CCA分析,表明优势属群(Bacillus、Lactococcus、Pseudomonas、Oceanobacillus、Stenotrophomonas、Psychrobacter、Myroides、Brochothrix和Arthrobacter)和超盐样本的聚集,与环境因子(总盐度、Cl^-、K^+、Mg^(2+)和CO_3^(2-)浓度)呈明显的正相关.
The bacterial structure and diversity of extremely salty lakes on the Qinghai-Tibet Plateau were investigated using high-throughput sequencing approach based on16S rRNA gene(V3~V4region),and then the difference of community compositions and the restrict correlation of hypersaline environmental factors were investigated.Results showed that the bacterial communities of hypersaline samples were relatively stable and evolutionally convergent,and the complexity and the diversity index were significantly lower than the usual salt environment.The dominant bacterial phyla were affiliated to Firmicutes(74.04%to81.04%of relative abundance proportion),followed by Proteobacteria(15.51%to20.06%),Bacteroidetes(2.68%to4.84%)and Actinobacteria(0.71%to2.45%).Base on the comparative analysis of other types of hypersaline lakes,the characteristic genera in this study were Bacillus(50.63%to58.35%),Lactococcus(9.28%to11.81%)and Oceanobacillus(8.41%to10.52%).Canonical correspondence analysis showed that the dominant bacterial genera(including Bacillus,Lactococcus,Pseudomonas,Oceanobacillus,Stenotrophomonas,Psychrobacter,Myroides,Brochothrix and Arthrobacter)and the hypersaline samples cluster was positively correlated with the saline environment factors including total salinity,Cl-,K+,Mg2+,and CO32-.
作者
朱德锐
韩睿
石晴
沈国平
龙启福
双杰
ZHU De-rui;HAN Rui;SHI Qing;SHEN Guo-ping;LONG Qi-fu;SHUANG Jie(Research Center of Basic Medical Sciences, Qinghai University Medical College, Xining 810016, China;Qinghai Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University,Xining 810016, China)
出处
《中国环境科学》
EI
CAS
CSSCI
CSCD
北大核心
2017年第12期4657-4666,共10页
China Environmental Science
基金
国家自然科学基金资助项目(31560039)
青海省自然科学基金资助项目(2015ZJY23,2015ZJ929Q)
关键词
青藏高原
极端盐湖
嗜盐细菌
超盐环境
环境因子
Qinghai-Tibet Plateau
salt lake
halophilic bacteria
hypersaline environment
environmental factors
作者简介
朱德锐(1979-),男,湖北鹤峰人,副教授,博士,主要从事极端盐湖环境微生物研究.发表论文10余篇.;责任作者, 副研究员, hanrui11473@163.com
