摘要
为了探讨外源激素对桃儿七愈伤组织诱导的影响,以桃儿七的胚及无菌苗的子叶和胚轴为材料,以MS为基本培养基,研究了3种不同激素配比对不同外植体愈伤组织诱导的影响。结果表明,以胚为外植体,在3.0 mg/L 2,4-D、0.5 mg/L KT和0.5 mg/L IAA组合处理的培养基中,诱导率达到39.5%;诱导子叶和胚轴形成愈伤组织的最佳激素配比为4.0 mg/L 2,4-D+0.5 mg/L KT+0.2 mg/L IAA;成熟胚比子叶和胚轴更易诱导愈伤组织的形成,诱导率分别提高了43.64%和315.79%,诱导效果最显著(P<0.001);在接种前,用5mg/L 2,4-D溶液浸蘸子叶和胚轴可有效提高其出愈率。
Embryos and cotyledon and hypocotyl of plantlets from Podophyllum hexandrum royle were used to establish callus cultureswith MS medium with various hormones in order to study the effects of hormone on callus induction.The results indicated that theembryos callus induction rate could reach39.5%under the interactions treatments of3.0mg/L2,4-D+0.5mg/L KT+0.5mg/L IAA.4.0mg/L2,4-D+0.5mg/L KT+0.2mg/L IAA was the optimal hormone ratio selected for the cotyledon and hypocotyl callus induction.Matureembryos were more likely to induce callus formation than cotyledons and hypocotyls,which were43.64%and315.79%higher than thoseof the two.The induction effect was the most significant(P<0.001).The dipping of5mg/L2,4-D for cotyledon and hypocotyl before thevaccination was increased the callus induction rate effectively.
作者
李金娟
陶自为
陶海霞
李婷
LI Jin-juan;TAO Zi-wei;TAO Hai-xia;LI Ting(Institute of Quality Standards and Testing Technology, GanSu Academy of Agricultural Sciences, Lanzhou 730070, PRC;Information Technology Services of LanZhou University, Lanzhou 730050, PRC)
出处
《湖南农业科学》
2017年第9期6-9,共4页
Hunan Agricultural Sciences
关键词
外源激素
桃儿七
愈伤组织
Hormone
Podophyllum hexandrum royle
callus
作者简介
李金娟（1985-），女，甘肃兰州市人，研究实习员，主要从事植物发育生物学相关研究
