摘要
为开发高效安全的对虾黑变抑制剂,研究3种巯基化合物对凡纳滨对虾(Litopenaeus vannamei)多酚氧化酶(Polyphenol oxidase,PPO)的抑制效果和动力学。结果表明:当L-半胱氨酸、N-乙酰-L-半胱氨酸和还原性谷胱甘肽质量浓度分别为0.4、0.8、1.6 mg/mL时,与PPO作用5 min,对PPO的抑制率分别达85.3%、89.3%、91.2%;3种巯基化合物质量浓度为0~0.8 mg/mL时对凡纳滨对虾PPO的抑制为竞争性抑制,质量浓度为0.8~6.4mg/mL时的抑制为不可逆抑制。3种巯基化合物对凡纳滨对虾PPO的抑制是两种机制并存。
In order to develop safe and effective inhibitors of shrimp melanosis,inhibition effects and kinetics of three sulfhydryl compounds on polyphenol oxidase from Litopenaeus vannamei were studied.When the concentrations of L-cysteine,N-acetyl-L-cysteine and reduced glutathione were0.4,0.8and1.6mg/mL and reacted with PPO for5min,the inhibition rates were85.3%,89.3%and91.2%,respectively.Electrophoresis and inhibition kinetics revealed that the sulfhydryl compounds showed a competitive inhibition at the concentrations of0-0.8mg/mL and showed an irreversible inhibition at the concentrations of0.8-6.4mg/mL.Therefore,the two inhibitory mechanisms of three sulfhydryl compounds on PPO may be coexistent.
作者
刘蒙娜
刘媛
刘书成
吉宏武
高静
郝记明
毛伟杰
LIU Meng-na;LIU Yuan;LIU Shu-cheng;JI Hong-wu;GAO Jing;HAO Ji-ming;MAO Wei-jie(College of Food Science and Technology, Guangdong Ocean University // Key Laboratory of Aquatic Products Processing and Safety // Guangdong Provincial Seafood Engineering Technology Research Center // Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institut)
出处
《广东海洋大学学报》
CAS
2017年第3期67-72,共6页
Journal of Guangdong Ocean University
基金
国家自然科学基金项目(31371801)
广东省科技计划项目(2015A020209158)
现代农业产业技术体系专项基金(CARS-47)
关键词
凡纳滨对虾
多酚氧化酶
抑制剂
巯基化合物
抑制动力学
Litopenaeus vannamei
polyphenol oxidase
inhibitor
sulfhydryl compounds
inhibition kinetics
作者简介
刘蒙娜（1990—），女，硕士研究生，研究方向为水产品保鲜与加工。;通信作者：刘书成（1977—），男，博士，教授，研究方向为水产品保鲜与加工。Lsc771017@163.com
