摘要
目的运用液相色谱-质谱法测定茜草药材中芦西丁的含量。方法采用Agilent1290HPLC-G6460三重四级杆质谱仪，在ESI-离子模式下选择离子监测，色谱分离采用AgilentEclipsePlusC18RRHD色谱柱（2.1mm×50mm，1.8μm），以0.1%甲酸-乙腈（70∶30）为流动相，柱温30℃，流速0.4mL/min，进样量2μL。结果芦西丁在0.0052～2.64ng范围内呈良好线性关系（r＝0.9999），80%、100%、120%浓度水平的加样回收率为94.80%～100.94%（平均97.17%，RSD＝6.16%），方法重复性及精密度均良好。结论本研究建立的方法灵敏、准确，重复性好，适用于茜草中芦西丁的含量测定，可用于茜草药材中该成分的限度检查。
Objective To develop a LC-MS method for the content determination of lucidin in Rubiae Radix et Rhizoma. Methods Agilent 1290 HPLC and G6460 Mass were used for quantification in MRM mode under ESI-.chromatography was performed on a Agilent Eclipse Plus C18 RRHD column (2.1 mm × 50 mm, 1.8 μm) at 30 ℃ using acetonitrile-0.1% formic acid in water at the flow rate of 0.4 mL/min. The injection volume was 2 μL.Results Good linearity was obtained from concentration of lucidin among the range of 0.005 2–2.64 ng (r= 0.999 9).Recoveries at different concentrations (80%, 100%, 120%) were between 94.80% and 100.94% (AVE=97.17%,RSD=6.16%). Good reproducibility and precision were also achieved. Conclusion The new developed LC-MS method is sensitive, accurate and with good repeatability, which is suitable for the content determination of lucidin in Rubiae Radix et Rhizoma. It can be used in the limit detection of lucidin in Rubiae Radix et Rhizoma.
作者
浦益琼
丁越
王冰
张若曦
张彤
PU Yi-qiong;DING Yue;WANG Bing;ZHANG Ruo-xi;ZHANG Tong(Shanghai University of Traditional Chinese Medicine,Shanghai 201203, China)
出处
《中国中医药信息杂志》
CAS
CSCD
2016年第12期96-98,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
上海市科学技术委员会科研计划项目(11DZ1971500)
上海市卫生局青年科研项目(20124Y002)
作者简介
通讯作者：张彤，E-mail：zhangtdmj@hotmail.com
