摘要
目的:探讨丙型肝炎酶联免疫吸附试验(ELISA)法和荧光定量PCR HCV-RNA检测血清中抗-HCV与HCV-RNA的结果。方法:采集102例慢性丙肝患者的血清,用ELISA法和荧光定量PCR HCV-RNA法进行检测。结果:102例患者中,抗-HCV(+)组74例,检出率72.5%(74/102),抗-HCV(-)组28例,检出率27.5%(28/102),在HCV-RNA检测,74例中54例病毒含量≥104 IU/mL,检出率73.0%(54/74),28例中3例病毒含量≥104 IU/mL,检出率10.7%(3/28),两组比较,差异有统计学意义(P<0.05)。结论:联合检测抗-HCV与HCV-RNA可提高丙肝患者的检出率,通过测定结果的对比,抗-HCV诊断丙肝患者有漏诊现象,荧光定量PCR HCV-RNA的特异性高,敏感度强,是临床诊断丙型肝炎的重要依据。
Objective:To explore the results of ELISA method and fluorescence quantitative PCR HCV-RNA for detection ofanti-HCV and HCV-RNA in serum in hepatitis C.Methods:Serum of 102 patients with chronic hepatitis C was collected.Theywere detected by ELISA method and fluorescence quantitative PCR HCV-RNA.Results:In 102 patients,74 cases were inanti-HCV(+) group,28 cases were in anti-HCV(-) group,and the detection rate was 27.5%(28/102).In HCV-RNA detection,in 74cases,the virus content of 54 cases were more than 104 IU/mL,and the detection rate was 73%(54/74);in 28 cases,the virus contentof 3 cases were more than 104 IU/mL,and the detection rate was 10.7%(3/28);there was significant difference between groups(P<0.05).Conclusion:The combined detection of anti-HCV and HCV-RNA can improve the detection rate of hepatitis C patients.Bycomparing the results of the test results,anti-HCV had misdiagnosis phenomenon in the diagnosis of hepatitis C patients,influorescence quantitative PCR HCV-RNA,the specificity was high,and sensitivity was strong.It was an important basis for clinicaldiagnosis of hepatitis C.
作者
周亚丽
朱文克
Zhou Yali;Zhu Wenke(Clinical Laboratory,the People's Hospital of Xiangcheng County,Henan Province 461700)
出处
《中国社区医师》
2016年第18期142-143,共2页
Chinese Community Doctors
