摘要
应用稳定表达ＣＹＰ２Ｂ６的转基因细胞系ＣＨＬ－２Ｂ６对一组前致突变物／致癌物进行双核微核试验，同时以母细胞系ＣＨＬ为对照，结果表明：５种前致突变物ＮＮＫ、ＤＥＮ、ＮＭ、ＭＢＩ和ＣＰ均能诱导ＣＨＬ－２Ｂ６细胞的徽核率显著增加，其中ＮＮＫ、ＣＰ和ＡＦＢ１具有剂量－反应关系，在最高受试浓度微核率约增加３～８倍。显示转基因细胞系ＣＨＬ－２Ｂ６在化学致癌物的遗传毒理学研究方面具有重要的应用价值．
The study reported the bioactivation of a panel of promutagens [4-methylnitrosaminry-1-(3-pyridyl)-1-butanone (NNK), N-nitrosodiethylamine (DEN), Nitrosomorpboline (NM), Anatoxin B1 (AFB1) and Cyclophosphamide (CP)] in CHL cell line and genetically engineered immortal CHL-2B6 cell line expressing human CYP2B6 cDNA. The end point selected for study was the induction of micronucleu. All chemicals tested induced micronucleu repeducebly. The consistent dose-response relations were obtained in cultures treated with NNK, AFB1 and CP, which caused approximately 3～8 fold increase in micronucleus frequency over controls at the highest test concentration This study demonstrates that the use of genetically engineered immortal CHL-2B6 cell line expressing human CYP2B6 cDNA in vitro short term mutagenicity assay and in vitro studies of metabolic pathwsy carried out by the enzyme encoded by CYP2B6 gene Potentially provides a Powerful system for the eveluation of related chemicals.
基金
国家自然科学基金
