摘要
目的:探讨白介素12和γ干扰素对于急性白血病患者的检测价值。方法:收集2012年1月至2015年2月我院收治的76例初发而且未经治疗的AL患者的临床资料。选取同时期来我院进行健康体检的40例健康者作为对照组。采取ELISA双抗体夹心法检测患者IL-12和IFN-γ水平,并分析检测结果。结果:未治组AL、ALL和ANLL患者血清IL-12和IFN-γ水平均明显低于对照组(P<0.05),未治组ALL和ANLL患者血清IL-12和IFN-γ水平无显著性差异(P>0.05)。AL治疗后CR患者血清IL-12和IFN-γ水平均明显高于治疗前水平(P<0.05),但与对照组无显著性差异(P>0.05)。AL治疗后NR患者血清IL-12和IFN-γ水平均明显低于对照组(P<0.05),并与治疗前水平无显著性差异(P>0.05)。AL-CR、AL-NR的患者治疗前血清IL-12和IFN-γ水平无显著性差异(P>0.05)。AL-CR患者治疗后血清IL-12和IFN-γ水平明显高于AL-NR患者(P<0.05)。未治ALL组中各免疫分型之间血清IL-12和IFN-γ水平差异不显著(P>0.05)。未治AL组患者临床危险度分组之间血清IL-12水平差异不显著(P>0.05)。未治AL组患者临床危险度分组之间血清IFN-γ水平差异显著(P<0.05)。AL未治组患者血清IL-12水平与血清IFN-γ水平之间有相关性(r=0.735,P<0.05)。AL-CR组IL-12与IFN-γ二者的血清水平之间无明显相关性(r=0.292,P>0.05)。结论:AL患者IL-12和IFN-γ水平均较低,临床可通过其水平变化辅助AL的诊治。
Objective: To explore the diagnostic values of interleukin-12( IL-12) and interferon-γ( IFN-γ) for the patients w ith acute leukemia( AL). Methods: A total of 76 cases of AL w ere enrolled in this study,and the 40 healthy persons w ere used as control group. The levels of IL-12 and IFN-γ w ere determined by enzyme linked immunosorbent assay( ELISA). The results w ere analyzed. Results: The levels of IL-12 and IFN-γ in the untreated AL group,ALL and ANLL groups w ere low er significantly than those in the control group( P < 0. 05),there w as no significant difference betw een untreated AL and ANLL groups( P > 0. 05). The levels of IL-12 and IFN-γ in CR patients of AL group after treatment obviously higher than that of patients before treatment( P < 0. 05),but there w as no significant difference as campared w ith that in control. The levels of IL-12 and IFN-γ in NR patients of AL group after treatment w ere obviously low er than that in control group( P < 0. 05),but there w as no significant difference in comparision w ith patients before treatment( P > 0. 05). The levels of IL-12 and IFN-γ of AL-CR and AL-NR patients before treatment w ere not significant difference before treatment( P > 0. 05). The levels of IL-12 and IFN-γ of AL-CR patients obviously higher than that in AL-NR patients( P < 0. 05). According to immure classification,the levels of IL-12 and IFN-γ of patients in untreated group w ere not significant difference. In regard to the clinical risk degree,the level of IL-12 of patients in untreated group w as not obvious difference( P > 0. 05),but the level of IFN-γ of patients in untreated group w as obvious different( P < 0. 05). The level of IL-12 of patients in untreated group positively correlated w ith level of IFN-γ( r = 0. 735,P < 0. 05),but the level of IL-12 did not significantly correlated with the level of IFN-γ( r = 0. 292,P >0. 05). Conclusion: The serum levels of both IL-12 and IFN-γ are low er,but the changes of both serum levels may be helpful to diagnose and treatment of AL patients.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2016年第3期707-711,共5页
Journal of Experimental Hematology
