摘要
目的 探讨核心蛋白聚糖 (decorin)对正常肝细胞和肝星形细胞诱导凋亡的作用。方法 以正常人肝细胞株 (L 0 2细胞 )和大鼠肝星形细胞株 (HSC T6细胞 )为研究靶细胞 ,通过HE染色、流式细胞仪分析、TUNEL标记和电镜观察等方法证实decorin对肝细胞和肝星形细胞凋亡的诱导作用。结果 Decorin能够诱导两种靶细胞出现典型的凋亡细胞特征 ,细胞核浓缩、边聚 ,胞膜皱褶 ,凋亡小体形成。而且其诱导凋亡作用呈现剂量和时间效应。结论 Decorin能够诱导肝细胞和肝星形细胞凋亡 ,提示可以通过凋亡途径减少因损伤刺激而过度增生的细胞 。
Objective To reveal the effect of decorin in inducing normal hepatocytes and hepatic stellate cells (HSC) to apoptosis.Methods Both the two cell lines were cultured with decorin,the apoptosis was detected by observing the morphologic changes,analyzing the content of DNA by flowcytometry (FCM),TUNEL DNA-cleavage and by transmission electron-microscopy to identify the characteristics of apoptosis.Results Apoptosis of L-02 and HSC-T6 cells could be efficiently induced by decorin (20～40 mg/L),with the presence of apoptotic changes in morphology,apoptotic peak before G 1 phase of cell cycle on FCM.Under the microscope,cells displayed a characteristic pattern of structural changes in membrane and cytoplasm,including rapid blebbing of plasma membrane and nuclear disintegration,chromatin condensation and apoptotic body formation,but negative to trypan blue staining.Apoptosis of these cells showed a time-dose relationship.Conclusion Apoptosis of L-02 and HSC-T6 cells could be efficiently induced by decorin.This might be useful for the treatment of liver fibrosis.
出处
《中国药物与临床》
CAS
2002年第5期277-280,I001,共5页
Chinese Remedies & Clinics
基金
国家自然科学基金资助项目 ( 396 0 0 0 6 7)
