摘要
本研究首次以盐生草(Halogeton glomeratus)为材料,模拟盐胁迫(100mmol·L^(-1) NaCl)环境,探讨不同浓度重金属Cu^(2+),Zn^(2+),Ni ^(2+),Cd^(2+),Pb^(2+)处理对其萌发特性的影响。结果表明:随处理浓度的升高,发芽率和发芽势均表现为先升高后下降趋势,表明低浓度重金属离子可促进植物的萌发,幼苗鲜重、干重、株高均呈现逐渐下降趋势。测定离子含量得出,Cd^(2+)和Pb^(2+)离子含量随着胁迫加重呈先升高后下降的趋势,Cu^(2+),Zn^(2+)和Ni^(2+)离子含量逐渐增加。测定根系活力发现,Cu^(2+),Zn^(2+)根系活力随浓度的升高呈先上升后下降趋势,Ni^(2+),Cd^(2+),Pb^(2+)根系活力逐渐降低。综合聚类及主成分分析得出:盐生草耐Cu^(2+),Zn^(2+),Ni^(2+),Cd^(2+),Pb^(2+)的临界浓度分别为:1.00mmol·L^(-1),10.00mmol·L^(-1),0.30mmol·L^(-1),0.20mmol·L^(-1)和0.50mmol·L^(-1),在胁迫浓度达临界值时贡献率最大的指标分别为:萌发指标、干重、发芽势、干重和发芽势。
This study firstly researched germination characteristics of Halogetonglomeratusunder different concentrations of heavy metals Cu2+,Zn2+,Ni2+,Cd2+and Pb2+supplemented with 100 mmol·L-1 NaCl.The results showed that germination rate and germination potential increased at first and then decreased with the increase of the heavy metals concentrations.It indicated that the low concentration of heavy metal ions promoted the germination of plants.Fresh weight,dry weight and seedlings height decreased gradually.The results of ion contents showed that Cd2+and Pb2+contents increased at first and then decreased,and Cu2+,Zn2+and Ni2+contents increased with increasing heavy metals concentrations.The root activity of Cu2+and Zn2+increased first and then decreased,and it decreased with the increase of Ni2+,Cd2+and Pb2+concentrations.The comprehensive clustering and principal component analysis were carried out to obtain the critical concentrations of Cu2+,Zn2+,Ni2+,Cd2+and Pb2+in Halogeton glomeratus,which were1.00 mmol·L-1,10.00 mmol·L-1,0.30 mmol·L-1,0.20 mmol·L-1 and 0.50 mmol·L-1,respectively.The most important indexes were germination index,dry weight,germination energy,dry weight andgermination energy when the stress concentration reached the critical value for Halogeton glomeratus.
作者
侯静静
李葆春
汪军成
姚立蓉
边秀秀
胡娜
司二静
杨轲
孟亚雄
马小乐
王化俊
HOU Jing-jing;LI Bao-chun;WANG Jun-cheng;YAO Li-rong;BIAN Xiu-xiu;HU Na;SI Er-jing;YANG Ke;MENG Ya-xiong;Ma Xiao-le;WANG Hua-jun(Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement&Germplasm Enhancement,Lanzhou,Gansu Province 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China;College of life Sciences and Technology,Gansu Agriculture University,Lanzhou,Gansu Province 730070,China)
出处
《草地学报》
CAS
CSCD
北大核心
2019年第1期112-122,共11页
Acta Agrestia Sinica
基金
国家大麦青稞产业技术体系(CARS-05-03B-03)
甘肃省重大专项(17ZD2NA016)
甘肃农业大学学科建设专项基金(GAUXKJS-2018-082
083)
甘肃农业大学盛彤笙创新基金(GSAU-STS-2018-33)资助
关键词
重金属
胁迫
盐生草
萌发
Heavy metals
Stress
Halogeton glomeratus
Germination
作者简介
侯静静(1994-),女,甘肃兰州人,硕士研究生,主要从事作物遗传育种研究,E-mail:528725294@qq.com;李葆春(1971-),女,甘肃陇南人,副教授,博士,主要从事小麦遗传育种研究,E-mail:libc@gsau.edu.cn;通信作者Author for correspondence,E-mail:huajunwang@sina.com
