摘要
研究土样处理方法、土壤基质类型、土样稀释倍数(10^(-1)、10^(-2)、10^(-3)、10^(-4))等因子对纤维素降解真菌分离率的影响,得到提高纤维素降解真菌分离率的最佳方案。以西藏林芝的腐殖质土、沙质土为实验材料,采用稀释分离法分离纤维素降解真菌,统计不同处理下纤维素降解真菌的分离率。研究发现,将土样阴干后并研细可提高纤维素降解真菌的分离率;腐殖质土及100倍土壤稀释液分离效果好;碳源为CMC-Na,氮源为混合氮源的分离培养基分离率最佳;菌株培养温度为25℃时纤维素降解真菌的分离率最高。本试验为高效分离纤维素降解真菌提供了理论依据。
The influence of soil sample treatment method, soil matrix type, soil sample dilution times（10^（-1）, 10^（-2）, 10^（-3）, 10^（-4）） and other factors on the separation rate of cellulose-degrading fungi were studied in this paper in order to get the best scheme to improve the separation rate of cellulose-degrading fungi. Taking the humus soil and sandy soil of Nyingchi in Tibet as experimental materials, cellulose-degrading fungi were separated by dilution separation method, and the separation rates of cellulose-degrading fungi under different treatments were statistically analyzed. It was found that drying soil samples in the shade and grinding improved the separation rates of cellulose-degrading fungi. Humus soil and 100 times soil diluent had good separation effects. The separation medium with CMC-Na as carbon source and mixed nitrogen source as nitrogen source had the best separation rate. The isolation rate of cellulose-degrading fungi was the highest when the strain culture temperature was 25℃.This experiment provided a theoretical basis for the efficient separation of cellulose-degrading fungi.
作者
赵艳艳
岳海梅
李景江
刘燕
ZHAO Yanyan;YUE Haimei;LI Jingjiang;LIU Yan(Plant Sciences College,Tibet Agriculture ＆ Animal Husbandry University,Nyingchi Tibet,860000;QiaoNv Nature Conservation Cebter,Guazhou Gansu 736100;Academy of Animal and Veterinary Science,Qinghai University,Qinghai xining,810016,China)
出处
《高原农业》
2018年第4期366-372,426,共8页
Journal of Plateau Agriculture
基金
国家自然科学基金(31760006)
作物学科建设项目(2015ZWXKJS
2016ZWXKJS)
西藏农牧学院研究生创新项目(YJS2018-19)
关键词
纤维素降解真菌
培养基
分离
筛选
Cellulose-decomposing fungi
culture medium
separation
screening
作者简介
赵艳艳（1995-）,女,藏族,甘肃临潭人,在读硕士.研究方向：主要研究高原植物病理学方面的内容.;通讯作者：岳海梅（1979-）,女,汉族,新疆和静人,副教授.研究方向：主要从事高原植物病理学方面的研究.
