摘要
目的乙型肝炎病毒(HBV)基因型及耐药突变情况与疾病进展和药物疗效密切相关,有效开展其测定具有重要意义。文中旨在检测桂西地区869例HBV的基因型及耐药突变基因,为临床抗HBV治疗提供依据。方法收集2016年1月至2018年3月右江民族医学院附属医院感染性疾病科门诊及住院的来自桂西地区且HBV DNA>1.0×103IU/mL的869例慢性乙型肝炎病毒携带(CHB)患者。采用PCR和反向点杂交法技术检测其HBV基因分型与耐药突变情况。结果共检出7种基因分型,分别为B基因型304例(34.98%),C基因型268例(30.84%),D基因型147例(16.92%)及B+C基因型、B+D基因型、C+D基因型、B+C+D基因型。63例患者发生耐药突变,耐药突变率为7.25%,其中C基因型31例(49.21%),B基因型19例(30.16%);突变模式有rt204I、rt181V、rt236T、rt180M+rt204V+rt204I等14种,其中rt180M+rt204V突变频率最高(17.46%)。B、C基因型的总体突变率(19%、31%)差异有统计学意义(P<0.05)。结论桂西地区CHB患者HBV基因型多样,主要以B、C基因型为主,且耐药突变率较高,突变模式复杂,这一结果为桂西地区临床抗HBV治疗提供了依据。
Objective The genotypes and drug-resistance mutation of hepatitis B virus （HBV） are closely related to the progression of chronic hepatitis B （CHB） and effects of medication. This study was to identify the genotypes and drug-resistance mutation genes of HBV in West Guangxi and provide some laboratory evidence for anti-HBV treatment. Methods We collected serum samples from 869 CHB patients with HBV DNA 〉1.0×10^3 IU/mL, all from West Guangxi, between January 2016 and March 2018. We detected the genotypes and drug-resistance mutation genes in the patients by PCR and reverse dot blot （PCR-RDB）. Results A total of 7 genotypes were identified in the patients, including genotypes B in 304 cases （34.98%）, C in 268 （30.84%）, D in 147 （16.92%）, B＋ C in 28 （3.22%）, B＋D in 22 （2.53%）, C＋D in 1 （0.12%） and B＋C＋D in 1 （0.12%）. Drug-resistance mutation was observed in 63 cases （7.25%）, including 31 cases with genotype C （49.21%） and 19 with genotype B （30.16%）. Fourteen gene mutation patterns were found in all, including rt204I, rt181V, rt236T, rt180M＋rt204V, and rt180M＋rt204V＋rt204I, among which, rt180M＋rt204V exhibited the highest mutation frequency （17.46%）. There was a statistically significant difference between the mutation rates of genotypes B and C （19% vs 31%, P 〈 0.05）. Conclusion CHB patients in West Guangxi have a wide variety of genotypes of HBV, mainly including genotypes B and C, with a high rate and complicated patterns of drug-resistance mutation, which has provided some reference for anti-HBV treatment in West Guangxi.
作者
许桂丹
肖树荣
王春芳
韦武均
彭彬
邓益斌
XU Gui-dan;XIAO Shu-rong;WANG Chun-fang;WEI Wu-jun;PENG Bin;DENG Yi-bin(Department of Laboratory Center;Department of Infectious Diseases Center,Affilated Hospital of You Jiang Medical College for Nationalities,Baise 533000,Guangxi,China)
出处
《医学研究生学报》
CAS
北大核心
2018年第11期1163-1166,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(81460123)
广西壮族自治区研究生创新项目(YCSW2017216)
作者简介
医学硕士研究生;通信作者：邓益斌,E-mail：dengyb75＠163.com
