摘要
目的：研究小叶九里香Murraya microphylla枝干的化学成分并进行分离鉴定,对分到的化合物进行体外抑制8种植物病原真菌活性研究。方法：采用常压硅胶柱色谱,半制备高效液相色谱,LH-20型羟丙基葡聚糖凝胶（Sephadex LH-20）等柱色谱方法对其枝干的甲醇提取物进行系统分离纯化,通过^1H-NMR,^13C-NMR,MS等方法进行化合物的鉴定结构;采用菌丝生长速率法对得到的化合物进行体外抑制8种植物性病原真菌的活性测定。结果：分离得到11个化合物,分别鉴定为girinimbine（1）,mahanimbine（2）,koenimbine（3）,koenidine（4）,mukonal（5）,murrayanine（6）,murrayaquinone-B（7）,β-sitosterol（8）,daucosterol（9）,3β-hydroxystigmast-5-en-7-one（10）,tetracosanoic acid,methyl ester（11）;在200 mg·L^-1,1-7系列咔吧唑生物碱对测试的植物病原真菌具有不同程度的抑制活性,其中对水稻稻温病菌活性较好;murrayanine（6）和girinimbine（1）具有潜在高效的广谱活性,对水稻稻温病菌表现出高活性,其半抑制浓度（IC50）分别为65.81,157.46 mg·L^-1,可为此类抑菌药物的先导化合物。结论：化合物2-3,5-7,10-11均为首次从小叶九里香中分离得到。murrayanine（6）和girinimbine（1）有进一步研究与开发的潜力。
Objective： To investigate the chemical compositions from the stems of Murraya microphylla and their anti-fungal activity against eight plant fungal pathogens in vitro. Method： The compounds were isolated and purified by the normal phase silica gel,Sephadex LH-20 HPLC column chromatography and other methods.Their structures were identified on the basis of spectral analysis such as ^1H-NMR,^13C-NMR and MS. Their antifungal activity was evaluated by the mycelium growth rate method. Result： Eleven compounds were obtained and identified as girinimbine（ 1）, mahanimbine（ 2）, koenimbine（ 3）, koenidine（ 4）, mukonal（ 5）,murrayanine（ 6）,murrayaquinone-B（ 7）,β-sitosterol（ 8）,daucosterol（ 9）,3β-hydroxystigmast-5-en-7-one（ 10）,and tetracosanoic acid,methyl ester（ 11）,respectively. Even at the high concentration of 200 mg·L^-1,carbazoles 1-7 exhibited different anti-fungal activity against these fungi in vitro, especially for M. grisea.Compounds 6 and 1 showed a broad spectrum and potent activity against these fungal pathogens,exhibiting a high activity against M. grisea,with IC50 values of 65. 81,161. 61 mg·L^-1,respectively. Conclusion： Compounds2-3,5-7 were isolated from the plant for the first time. Compounds 1 and 6 had great potentials for further research and development.
作者
卜淼淼
余森泉
董存柱
BU Miao-miao;YU Sen-quan;DONG Cun-zhu(Institute of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第23期69-76,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
海南省自然科学基金面上项目(317046)
海南省重点研发计划项目(ZDYF2016157)
国家自然科学基金项目(31360447)
关键词
小叶九里香
枝干
化学成分
咔吧唑生物碱
植物病原真菌
抑菌活性
Murraya microphylla
stems
chemical constituents
carbazole alkaloids
phytopathogen
antifungal activities
作者简介
[第一作者]卜淼淼，硕士，从事天然产物分离与活性物质筛选研究，E—mail：shwjeyyll@loulook．com;[通信作者]董存柱，副教授，硕士生导师，从事天然产物化学研究，E-mail：czd@hainu．edu．cn
