摘要
目的 探讨维生素D受体（VDR）基因多态性在肺癌筛查中的临床价值.方法 选择佛山市第五人民医院2017年1～9月行肺癌筛查人群2000例为研究对象,VDR基因多态性测定使用酚-氯仿法进行外周基因DNA提取,先以χ^2检验对不同VDR基因型者[Bsm1（bb及Bb）、Apal（aa、Aa及AA）]是否出现肺癌行单因素分析,确定其与肺癌之间的相关性,随后行VDR基因多态性与肺癌发生的多因素非条件logistic回归分析,探讨不同VDR基因型与肺癌发生的危险因素关系.结果 非肺癌者血清维生素D水平为（46.5±2.3）ng/L,肺癌者血清维生素D水平为（26.5±1.1）ng/L,非肺癌者血清维生素D水平显著高于肺癌者（t=49.614,P=0.000）.Bsm1位点bb基因型、Apal位点aa基因型和Apal位点Aa基因型为发生肺癌的相关危险因素,同时Bsm1位点bb基因型及Apal位点Aa基因型为发生肺癌的独立危险因素.结论 VDR中Bsm1位点bb基因型和Apal位点Aa基因型者是肺癌发生的高危人群,在临床初筛中应提高重视,避免误诊和漏诊.
Objective To investigate the clinical value of vitamin D receptor （ VDR） gene polymorphism in lung cancer screening .Methods From January 2017 to September 2017 ,2000 lung cancer screening patients in the Fifth People＇s Hospital of Foshan were selected in the research .The VDR gene polymorphism was determined by phenol-chloroform method .DNA was extracted from the peripheral blood of patients ,different VDR genotypes [ Bsm1 bb and Bb）,Apal（aa,Aa and AA）] were analyzed by univariate analysis to determine the correlation between lung cancer and VDR gene polymorphism and lung cancer incidence by multivariate non -conditional logistic regression analysis, thus to explore the relationship between different VDR genotypes and risk factors of lung cancer . Results The level of serum vitamin D in the non -lung cancer patients was （46.5 ±2.3） ng/L,which was signifi-cantly higher than （26.5 ±1.1）ng/L in the lung cancer patients （t=49.614,P=0.000）.The Bsm1bp genotype, Apal aa genotype and Apal Aa genotype were the risk factors of lung cancer .The Bsm1bp and Apal Aa genotypes were independent risk factors of lung cancer .Conclusion The Bsm1 locus and Apal locus Aa genotype in VDR receptor are high risk population of lung cancer .Therefore,we should pay more attention to the clinical screening and avoid misdiagnosis and missed diagnosis .
作者
叶文生
陈钢
盘德辉
陈仰新
余志辉
孟家晓
陈秀琼
Ye Wensheng;Chen Gang;Pan Dehui;Chen Yangxin;Yu Zhihui;Meng Jiaxiao;Chen Xiuqiong(Department of Internal Medicine,the Fifth People＇s Hospital of Foshan,Foshan,Guangdong 528000,China)
出处
《中国基层医药》
CAS
2018年第18期2320-2323,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
广东省佛山市卫生和计生局医学科研课题（20170148）
关键词
肺肿瘤
维生素D
受体
骨化三醇
基因多态性
Lung neoplasms
Vitamin D
Receptors,calcitriol
Gene polymorphism
