摘要
目的分析医院碳青霉烯耐药肺炎克雷伯菌(carbapenemase resistant K. pneumoniae,CRKP)尿路感染的分子流行病学特点,为临床科学防控提供实验室依据。方法选择2016年1-12月医院引起医院尿路感染的非重复CRKP菌株18株,采用多位点序列分析(MLST)方法进行分型,并使用PCR方法检测常见碳青霉烯酶基因(DNM、KPC、SME、VIM、GES、IMP、OXA-48)和多黏菌素耐药基因MCR-1等。结果 18株医院尿路感染CRKP菌株,KPC-2基因携带率为94.44%(17/18),其中一株同时携带KPC和MCR-1基因,MLST分型以ST11型为主(88.89%,16/18)。结论 CRKP引起的尿路感染患者,多年龄偏大,有保留导尿和高级别抗菌药物使用史,应该规范抗菌药物使用与加强消毒隔离措施,控制流行传播。
OBJECTIVE To analyze the clinical risk factors and molecular epidemiological characteristics of nosocomial CRKP infection in urinary tract,and to provide laboratory basis for clinical scientific prevention and control.METHODS The non-repetitive CRKP strains that caused urinary tract infection in a teaching hospital were collected from Jan.2016 to Dec. 2016,and were classified by multipoint sequence analysis（MLST）.The common carbapenem genes（DNM,KPC,SME,VIM,GES,IMP,OXA-48）and polymyxin resistance gene MCR-1 were detected by PCR method.RESULTS A total of 18 CRKP strains of nosocomial urinary tract infection were collected,and the KPC-2 gene carrying rate was 94.44%.One of them carried both KPC-2 and MCR-1 genes,and the MLST typing was dominated by ST11 type（88.89%,16/18）.CONCLUSION The patients with urinary tract infection caused by CRKP were usually at an old age,with a history of retention catheterization and use of highgrade antibiotics,and most of the CRKP strains that caused infection were type ST11 Klebsiella pneumoniae with KPC-2 type carbapenenes gene.It is necessary to standardize drug resistance and strengthen disinfection and isolation measures to control epidemic spread.
作者
孙丹
姜飞
樊慧丽
杨荣胜
康海全
SUN Dan;JIANG Fei;FAN Hui-li;YANG Rong-sheng;KANG Hai-quan(Xuzhou Children＇s Hospital,Xuzhou,Jiangsu 221002,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2018年第20期3041-3044,共4页
Chinese Journal of Nosocomiology
基金
国家自然科学基金资助项目(81471994)
徐州市科技计划基金资助项目(KC16SH019)
作者简介
通信作者：康海全，E-mail：hqk811029@163．com
