司帕沙星对类风湿关节炎成纤维样滑膜细胞的声动力细胞毒性被引量：1

Sonodynamic cytotoxicity of sparfloxacin on rheumatoid arthritis fibroblast-like synoviocytes in vitro

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摘要目的探讨司帕沙星介导声动力疗法对类风湿关节炎成纤维样滑膜细胞的损伤作用,并阐明其可能的作用机制。方法人类风湿关节炎成纤维样滑膜细胞MH7A经司帕沙星(浓度1.00μmol/L)处理后,采用超声辐照(声强1.0 W/cm^2,辐照60 s)。实验分为对照组、司帕沙星组、超声组和声动力(司帕沙星+超声)组。MTT法和流式细胞仪检测MH7A细胞的损伤情况。多功能荧光酶标仪测定细胞内活性氧物质产生情况。结果声动力组细胞活力显著低于对照组(P <0. 01),细胞凋亡率显著高于对照组(P <0. 01)。与对照组相比,声动力疗法显著地诱导MH7A细胞内产生过量活性氧物质(P <0. 01)。与活性氧物质清除剂甘露醇相比,活性氧物质清除剂组氨酸有效地阻断声动力疗法诱导MH7A细胞活力降低(P <0. 01)、细胞凋亡增加(P <0. 01)以及细胞内活性氧物质产生(P <0. 01)。结论司帕沙星介导声动力疗法显著地诱导MH7A细胞损伤,其机制可能与诱导细胞内产生过量活性氧物质有关。 ObjectiveTo investigate the impairing effect of sparfloxacin （SPFX）mediated sonodynamic therapy （SDT） on rheumatoid arthritis fibroblastlike synoviocytes and further explore its potential mechanism. MethodsHuman rheumatoid arthritis fibroblastlike synoviocyte cell line MH7A was incubated with 1.00 μmol/L SPFX, and then the cells were exposed to ultrasound irradiation （1.0 W/cm^2, 60 s）. The cells were randomly divided into control group, SPFX group, ultrasound group and SDT （SPFX＋ ultrasound） group. The viability of cells was examined by MTT assay. Cell apoptosis was analyzed using flow cytometry. The production of intracellular reactive oxygen species （ROS） was detected by fluorescence microplate reader. ResultsSDT resulted in significantly lower cell viability （P〈0.01）, higher apoptotic rate （P〈0.01）, and excessive intracellular ROS （P〈0.01） in MH7A cells when compared with the untreated control cells. Moreover, compared with ROS scavenger D-mannitol, ROS scavenger L-histidine could significantly inhibit the loss of cell viability （P〈0.01）, increase of cell apoptosis （P〈0.01） and production of intracellular ROS （P〈0.01） induced by SPFX-mediated SDT. ConclusionSPFX-mediated SDT shows cytotoxic effects on MH7A cells, which may be related to the excessive production of intracellular ROS.

作者田中华朱深银常淑芳李雪梅唐琴郑晓英郝兰王志刚 TIAN Zhonghua1, ZHU Shenyin1, CHANG Shufang3 , LI Xuemei1 , TANG Qin1 , ZHENG Xiaoying1 , HAO Lan2, WANG Zhigang2(1Department of Pharmacy, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016; 2Institute of Ultrasound Imaging, Chongqing Medical University, Chongqing, 400010; 3 Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China)

机构地区重庆医科大学附属第一医院药学部重庆医科大学附属第二医院妇产科重庆医科大学超声分子影像研究所

出处《第三军医大学学报》 CAS CSCD 北大核心 2018年第19期1748-1752,1761,共6页 Journal of Third Military Medical University

基金国家自然科学基金面上项目(81572558),重庆市研究生科研创新项目(CYS16127)

关键词司帕沙星声动力疗法成纤维样滑膜细胞细胞毒性活性氧物质 sparfloxacin sonodynamic therapy fibroblast-like synoviocytes eytotoxieity reactiveoxygen species

分类号 R593.22 [医药卫生—内科学] R454.3 [医药卫生—治疗学]

作者简介 [通信作者]朱深银，E-mail：zhushenyin0486@sina．com