摘要
以荧光标记为手段,利用激光共聚焦显微镜研究了不同浓度牛血清蛋白(BSA)及不同时间下,BSA在镁表面的吸附情况。采用扫描电子显微镜(SEM)、能谱仪(EDS)和X射线衍射(XRD)对吸附前后镁表面组织结构进行表征。通过动电位极化曲线测量与电化学阻抗谱方法研究了蛋白质吸附对镁表面抗腐蚀性能的影响。结果表明:随BSA浓度的增加,蛋白质的吸附量增多。随作用时间延长,表面粗糙度增加,BSA吸附量增加,蛋白质膜厚度增加,在一定程度上起到保护镁试样的作用。但是蛋白质与镁表面络合很可能加速点蚀的发生,镁表面产生大量腐蚀产物,其主要成分为氧化镁。蛋白质吸附整体上说会降低镁表面的抗腐蚀性能,而且其降低程度很可能与表面蛋白膜结构有关。
The adsorption of bovine serum albumin（ BSA） on magnesium surface under different concentrations and time was investigated by the fluorescence labelling method combined with the means of laser confocal microscopy. The surface microstructure of magnesium before and after adsorption was characterized by using the scanning electron microscopy（ SEM）,the energy dispersive X-ray spectroscopy（ EDS） and the X-ray diffraction（ XRD）.The effect of protein adsorption on the corrosion behavior of magnesium was studied by potentiodynamic polarization measurements and electrochemical impedance spectroscopy. The results show that protein adsorption increases with BSA concentration. BSA adsorption also increases with extension of exposure time of magnesium in protein solution,which attributes to the increase of surface roughness. A thick protein film could protect magnesium from corrosion to a certain extent,but the surface complexation of protein and magnesium most likely accelerates pitting corrosion,as a result lots of corrosion products appear on the surface.Magnesium oxide is the main compontent of corrosion products. Corrosion resistance of magnesium surface generally reduces in BSA solution,and the extent of reduction is probably associated with the protein structure on the surface.
作者
赵丽丹
丁文强
闫宏光
王欣
唐宾
ZHAO Li-dan;DING Wen-qiang;YAN Hong-guang;WANG Xin;TANG Bin(Department of Materials Science and Engineering,Taiyuan University of Technology,Taiyuan 030024,China)
出处
《轻合金加工技术》
CAS
北大核心
2018年第9期59-65,共7页
Light Alloy Fabrication Technology
基金
纳米Fe3O4@Ag2O可控合成及其生物相容性研究(编号201601D021049)
纳米氧化银表面与蛋白质的相互作用(编号120504020202)
关键词
镁
牛血清蛋白
蛋白质吸附
表面结构
抗腐蚀性能
magnesium
bovine serum albumin
protein adsorption
surface microstruc-ture
corrosion resistance
作者简介
赵丽丹（1992-），女，河南周口人，硕士研究生。
