摘要
目的观察P16蛋白、P53蛋白、人附睾蛋白4(HE4)在卵巢浆液性肿瘤中的表达水平及其临床检测意义。方法回顾性选择河北省秦皇岛市妇幼保健院病理科2012年1月至2017年1月存档的53例浆液性卵巢癌石蜡标本作为观察组,选择50例良性上皮性卵巢肿瘤患者肿瘤组织作为良性组,选择50例已绝经子宫肌瘤正常卵巢组织作为对照组,均为手术切除后病理学检查证实;对三组病理组织的P16、P53、HE4蛋白的表达水平进行检测,同时分析上述3种指标与卵巢临床病理学特征之间的关系。结果 P16在观察组、良性组、对照组的阳性表达率分别为90.6%(48/53)、22.0%(11/50)、2.0%(1/50),P53在观察组、良性组、对照组的阳性表达率分别为50.9%(27/53)、8.0%(4/50)、0(0/50),HE4在观察组、良性组、对照组的阳性表达率分别为73.6%(39/53)、12.0%(6/50)、0(0/50)。观察组的P16、P53、HE4的表达水平均高于良性组和对照组(P<0.05);观察组中,P16在临床分期晚期(Ⅲ/Ⅳ期)与早期(Ⅰ/Ⅱ期)、组织学分级高级与低级的阳性表达率差异均无统计学意义(P>0.05),P53在组织学分级高级与低级的阳性表达差异具有统计学意义(P<0.05),但在临床分期晚期(Ⅲ/Ⅳ期)与早期(Ⅰ/Ⅱ期)的阳性表达率差异均无统计学意义(P>0.05),HE4在临床分期晚期(Ⅲ/Ⅳ期)与早期(Ⅰ/Ⅱ期)、组织学分级高级与低级的阳性表达率差异均具有统计学意义(P<0.05)。结论 P16、P53在卵巢浆液性肿瘤中均有较高的阳性表达,但P53的阳性表达更多见于高级别卵巢浆液性癌中;HE4的表达与卵巢浆液性肿瘤的临床分期、组织学分级均有较大相关性,可为临床诊断和评估卵巢浆液性肿瘤预后提供参考。
Objective To observe the expression level and clinical significance of P16 protein, P53 protein and human epididymal protein 4 （HE4） in patients with ovarian serous tumors. Methods A retrospective selection of 53 cases of serous ovarian cancer in this hospital during January 2012 to January 2017 had been carried out, and specimens of these 53 cases were listede as observation group, and 50 cases of benign ovarian tumor as benign group, 50 cases of postmenopausal uterine leiomyoma received surgical treatment for the normal ovarian tissues as control group. All these patients were confirmed by postoperative pathological examination, and clinical data were intact; the expression level of immune markers were detected in three different groups of ovarian tissue P16, P53 and HE4 proteins, and analysis of these 3 indicators and clinical pathological features of ovarian tumors. Results P16 in observation group, the positive expression rate of benign group and control group were 90.6% （48/53）, 22.0% （11/50）, 2.0% （1/50） and P53 in observation group, the positive expression rate of benign group and control group were 50.9% （27/53）, 8.0% （4/50）, 0 （0/50）respecvely, HE4 in observation group, the positive expression rate of benign group and control group were 73.6% （39/53）, 12.0% （6/50）, 0 （0/50）. In observation group, the expression levels of P16, P53 and HE4 were higher than those in benign group and control group （ P 〈0.05）. iIn observation group, P16 in the late clinical stage （Ⅲ/Ⅳ period） and early stage （Ⅰ/Ⅱ period）, histological grades were senior and junior, lymph node metastasis and no positive expression rate of lymph node metastasis, and the differences had no statistical significance （ P 〉0.05）, and P53 in histological grading of upper and lower positive expression, the difference was statistically significant （ P 〈0.05）, but in the late clinical stage （Ⅲ/Ⅳ period） and early stage （Ⅰ/Ⅱ period）, without lymph node metastasis and the positive expression rate of difference had no statistical significance （ P 〉005）, HE4 in the late clinical stage （Ⅲ/Ⅳ period） and early stage （Ⅰ/Ⅱ period）, histological grade senior and junior, with no lymph node metastasis and the difference in positive expression rate, and the difference had statistical significance （ P 〈0.05）. Conclusion P16 and P53 have high positive expression in ovary serous tumors, but positive expression of P53 is more common in high-grade oarian serous carcinoma. HE4 expression and the clinical stage, histological grading of ovarian serous tumour have significant correlation, lymph node metastasis can be used as a judgment of ovarian serous reference indicator of tumor invasion, metastasis and prognosis.
作者
邵长好
贺红梅
赵长燕
SHAO Chang-hao;HE Hong-mei;ZHAO Chang-yan(Department of Obstetrics and Gynecology;Department of Pathology, Qinhuangdao Women and Children＇s Health Hospital, Qinhuangdao Hebei 066000, China.)
出处
《临床和实验医学杂志》
2018年第20期2198-2201,共4页
Journal of Clinical and Experimental Medicine
基金
河北省秦皇岛市科技支撑计划项目(编号:201703A137)
关键词
卵巢浆液性肿瘤
P16
蛋白
P53
人附睾蛋白
4
阳性表达率
临床意义
Ovary serous tumor
P16 protein
P53 protein
Human epididymis 4
Positive expression rate
Clinical significance
