摘要
选取1年生油桐幼苗为试验材料,研究不同温度下油菜素内酯(BR)对油桐幼苗生长及生理特性的影响。结果表明:8℃和38℃2种不同温度胁迫下,2个油桐物种幼苗生长减缓,叶片及栅栏组织变薄,叶绿素含量和SPAD值下降,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性升高,可溶性糖和可溶性蛋白含量增加,丙二醛(MDA)含量上升。相同温度下,叶片喷施BR可以使油桐幼苗生长量增加,叶片及叶片栅栏组织加厚,叶绿素含量和SPAD值上升、POD和SOD活性增加,可溶性糖和可溶性蛋白含量提升,叶片相对含水率(RWC)增加,MDA含量降低。说明BR可以减轻温度胁迫对油桐叶片的抑制程度,增强油桐叶片的生理功能,提高叶片内各种抗氧化酶活性,降低MDA对细胞膜的毒害作用,进而增加油桐幼苗的抗热性和抗寒性,加快油桐幼苗有机物的积累,最终使生物量增加。
This study focused on the effects of Brassinolide（BR） on growth and physiological characteristics of Tung tree seedlings under different temperatures. The results showed that the growth of two Tung tree seedlings was slowed down leaf and palisade tissue became thinner, chlorophyll content and the value of SPAD decreased, the activity of peroxidase （POD） and superoxide dismutase （SOD） increased, soluble sugar and soluble protein content increased, and malondialdehyde （MDA） content increased under 8 ℃ and 38 ℃ . At the same temperature, spraying BR on leaves can increase the growth of Tung tree, thicken the leaf and leaf palisade, increase the content of chlorophyll and the value of SPAD, increase the activity of POD and SOD, increase the content of soluble sugar and soluble protein, increase the relative water content of leaves （RWC）, and reduce the content of MDA. The results showed that BR could reduce the inhibition of temperature stress on the leaves of Tung tree, enhance the physiological function of leaves of Tung tree, increase the activity of various antioxidant enzymes in the leaves, reduce the toxicity of MDA to the cell membrane, and then increase the heat resistance and cold resistance of the seedlings of Tung tree, accelerate the accumulation of machinery in the seedlings of Tung tree, and eventually increase the biomass.
作者
张帆航
李泽
谭晓风
李文莹
杨超臣
李艳丽
ZHANG Fanhang;LI Ze;TAN Xiaofeng;LI Wenying;YANG Chaochen;LI Yanli(Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees,ministry of Educatio;Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province,Central South University of Forest and Technology,Changsha 410004,Hunan,China)
出处
《经济林研究》
北大核心
2018年第3期17-24,共8页
Non-wood Forest Research
基金
"十三五"国家重点研发计划项目(2017YFD0601304)
关键词
千年桐
三年桐
油菜素内酯
温度胁迫
栅栏组织
Vernicia montana
Verniciafordii
brassinolide
temperature stress
palisade tissue
作者简介
张帆航，硕士研究生。;通信作者：谭晓风，教授，博士，博士研究生导师。E-mail：tanxiaofengcn@126．com
