摘要
[背景]探讨RT-PCR法应用于小肠结肠炎耶尔森菌(腹泻粪便中)检验的临床价值.[病例报告]采用随机的原则选取行小肠结肠炎耶尔森菌检验的腹泻患者100例(2017年4月—2018年4月),按照随机等量原则分为培养法组和RT-PCR法组,每组各为50例.两组分别应用相应方法进行检验,比较两组腹泻粪便中小肠结肠炎耶尔森菌的检查率.RT-PCR法组腹泻粪便中耶尔森菌的检查率显著高于培养法组(P<0.05).[讨论]RT-PCR法检验小肠结肠炎耶尔森菌(腹泻粪便中)的临床价值较高,效果满意.
BACKGROUND To study the clinical value of RT-PCR method on the detection of 50 cases of Yersinia enterocolitica（diarrhea stool）.CASE REPORTS 100 patients with diarrhea tested by Yersinia enterocolitica were selected on a random basis（April 2017-April 2018）.They were divided into culture method group and RT-PCR group according to the principle of random equivalence,50 cases in each group.The two groups were tested by corresponding methods,and the detection rate of Yersinia enterocolitica in the feces of the two groups was compared.The detection rate of Yersinia enterocolitica in diarrhoea feces of RT-PCR group was significantly higher than that in culture method group（P〈0.05）.DISCUSSION The RT-PCR method had higher clinical value and satisfactory effect in the detection of Yersinia enterocolitica（diarrhea feces）.
作者
周东梅
ZHOU Dongmei(Department of Central Laboratory,Affiliated Hospital of Yanbian University,Yanji 133000,Jilin,China)
出处
《延边大学医学学报》
CAS
2018年第2期142-143,共2页
Journal of Medical Science Yanbian University
关键词
耶尔森菌小肠结肠炎
培养法
RT-PCR法
Yersinia enterocolitica
culture method
reverse transcription polymerase chain reaction
作者简介
周东梅（1979-），女，检验师，研究方向为临床检验．
