摘要
OBJECTIVE The aim of the present study was to investigate the anti-tumor effect and mechanism of a novel compound,C_3C_(12)PPD,a bioactive unnatural ginsenoside by metabolically engi.neered yeasts based on a new UDP-glycosyl-transferase from Bacillus subtilis.METHODS MTT assay was used to analyze the anti-proliferation activity of C_3C_(12)PPD in vitro.The effect of anti-tumor activity was observed by mouse Lewis xenograft model in vivo.The effects of C_3C_(12)PPD on suppressing the angio.genesis and invasion of A549 cells were investigated in vitro using Transwell and tube formation assays.RNAseq was used to find tagets of C_3C_(12)PPD.Western blotting was performed to investigate the expres.sion level of proteins in tumor tissues treated with C_3C_(12)PPD.RESULTS C_3C_(12)PPD could inhibit the growth of lung cancer in vitro and in vivo.At the dosage of 10.0 mg · kg^(-1),C_3C_(12)PPD inhibited tumor growth by 40.0%(P<0.05) in tumor weight in mouse Lewis xenograft.The inhibition of tube formation was 77.5%(P<0.01) and 80.3%(P<0.01) following treatment with 1×10^(-4) and 2×10^(-4) mol·L^(-1) C_3C_(12)PPD for 5 h,whereas the proliferation of EA.hy926 cells was not influenced under the above concentrations.Under the concentrations of 1×10^(-4) mol·L^(-1),C_3C_(12)PPD inhibited invasive ability of A549 cells(P<0.05).The results of RNAseq susgested that antitumor activity of C_3C_(12)PPD were associated with epithelial-mesenchymal transition(EMT) and angiogenesis.Moreover,the proteins related to EMT,Raf/MEK/ERK and AKT/mTOR signal pathways were effected by C_3C_(12)PPD analysed by western blotting.CONCLUSION These data suggested that C_3C_(12)PPD was able to supress lung cancer through inhibit EMT,invision and angiogenesis.
OBJECTIVE The aim of the present study was to investigate the anti-tumor effect and mechanism of a novel compound,C3C（12）PPD,a bioactive unnatural ginsenoside by metabolically engi.neered yeasts based on a new UDP-glycosyl-transferase from Bacillus subtilis.METHODS MTT assay was used to analyze the anti-proliferation activity of C3C（12）PPD in vitro.The effect of anti-tumor activity was observed by mouse Lewis xenograft model in vivo.The effects of C3C（12）PPD on suppressing the angio.genesis and invasion of A549 cells were investigated in vitro using Transwell and tube formation assays.RNAseq was used to find tagets of C3C（12）PPD.Western blotting was performed to investigate the expres.sion level of proteins in tumor tissues treated with C3C（12）PPD.RESULTS C3C（12）PPD could inhibit the growth of lung cancer in vitro and in vivo.At the dosage of 10.0 mg · kg（-1）,C3C（12）PPD inhibited tumor growth by 40.0%（P〈0.05） in tumor weight in mouse Lewis xenograft.The inhibition of tube formation was 77.5%（P〈0.01） and 80.3%（P〈0.01） following treatment with 1×10（-4） and 2×10（-4） mol·L（-1） C3C（12）PPD for 5 h,whereas the proliferation of EA.hy926 cells was not influenced under the above concentrations.Under the concentrations of 1×10（-4） mol·L（-1）,C3C（12）PPD inhibited invasive ability of A549 cells（P〈0.05）.The results of RNAseq susgested that antitumor activity of C3C（12）PPD were associated with epithelial-mesenchymal transition（EMT） and angiogenesis.Moreover,the proteins related to EMT,Raf/MEK/ERK and AKT/mTOR signal pathways were effected by C3C（12）PPD analysed by western blotting.CONCLUSION These data suggested that C3C（12）PPD was able to supress lung cancer through inhibit EMT,invision and angiogenesis.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2018年第4期286-287,共2页
Chinese Journal of Pharmacology and Toxicology
作者简介
Coresponding author： Yan LI, E-mail： liyanxiao@imm.ac.cn, Tel： （010） 63169181
