摘要
目的评估老年高剂量四价流感病毒裂解疫苗（A＋B套苗）的免疫原性。方法采用江苏金迪克生物技术有限公司研发的四价流感病毒裂解疫苗，以小鼠为动物模型进行免疫原性试验，测定各组抗血凝素抗体滴度，比较高剂量A＋B套苗与其他组疫苗的免疫原性差别。结果血凝抑制试验结果表明，高剂量单次免疫组对四型抗原的血凝抑制滴度均高于标准剂量单次免疫组；高剂量A＋B套苗的研究中，除高剂量先B苗后A苗组中抗A3的血凝素抗体滴度未显著增加外，其他各组高剂量两次免疫组血凝素抗体滴度均显著高于标准剂量两次免疫组（P〈0.001）。同时发现，不论高剂量还是标准剂量的A＋B套苗组中，先B苗后A苗组中抗A型的血凝素抗体滴度明显高于先A苗后B苗组；先A苗后B苗组中，抗B型的血凝素抗体滴度明显高于先B苗后A苗组。在高剂量A＋B套苗两次免疫时间的间隔对抗血凝素抗体产生的影响试验中，7 d与10 d免疫间隔的免疫效果优于3 d。结论高剂量四价老年流感疫苗（A＋B套苗）分两次注射、免疫间隔7～10 d的方式免疫效果最好，在流感季节可根据A型和B型流感病毒流行监测结果，选择或改变先A苗后B苗或先B苗后A苗使用程序，以增加对人群的保护，是预防老年流感的新方法。
ObjectiveTo evaluate the immunogenicity of high-dose inactivated quadrivalent influenza serial vaccines （split virion） for elderly people.MethodsImmunogenicity assays on mouse as research animal model with inactivated quadrivalent influenza serial vaccines （split virion） were carried out. Then rates of seroconversion and geometric mean hemagglutination inhibition titers （GMTs） at day 21 after the last vaccination among those who received high-dose （HD） A＋ B influenza vaccine, were compared with those who received other vaccines by hemagglutination inhibition （HAI） test.ResultsThe result of HAI test showed in HD serial vaccine groups, GMTs for all kinds of HA in the two HD serial vaccine groups were significantly different from that of the two SD serial vaccine groups, respectively （P〈0.0001）, but not the titers for A3 in one B dose first group. Further- more, GMTs for strain A in one B dose first groups were significantly higher than that of one B dose first groups in both HD and SD groups, and vice versa in the GMTs for strain B. The result of the assay of the impact of different immunization intervals of HD serial influenza vaccine on immunogenicity indicated that the immunity responses in 7 or 10 d groups were higher than that in 3 d group.ConclusionsIt is a new method to prevent the flu for elderly by HD A＋ B serial influenza vaccine, whose HA dose per immunization was reduced into two injections, worked best when the immunization interval was 7-10 d. The protective immunity can be improved by selecting immune procedure of the serial vaccine according to the epidemic type surveillance of influenza virus A and B strains.
作者
余军
杨文彬
杨宪普
帅旗
赵越
张赟
朱实惠
蒋正东
吴建华
赵静
付建林
Yu Jun;Yang Wenbin;Yang Xianpu;Shuai Qi;Zhao Yue;Zhang Yun;Zhu Shihui;Jiang Zhengdong;Wu Jianhua;Zhao Jing;Fu Jianlin(Jiangsu GDK Biological Technology Co.,Lid,Taizhou 225300,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2018年第4期341-346,共6页
Chinese Journal of Experimental and Clinical Virology
关键词
老年人
流感疫苗
四价
高剂量
A+B套苗
Elderly adults
Inactivated Influenza vaccine
Quadrivalent
High-dose
A＋ B serial
作者简介
通信作者：杨文彬，Email：bam7565@163．com
