摘要
通过单因素实验和正交实验优化百蕊草总黄酮的醇提法提取工艺,确定最佳工艺条件为:提取温度70℃、料液比1∶40(g∶mL)、乙醇体积分数80%、提取时间4h,在此条件下,总黄酮提取率达到5.33%。该方法的精密度、稳定性、重复性良好,回收率为97.213%。
We optimized the ethanol extraction process of total flavonoids fromThesiumchinenseby singlefactor experiments and orthogonal experiments.The optimum extraction conditions are determined as follows：the extraction temperature of 70 ℃,the solid-liquid ratio of 1∶40（g∶mL）,the ethanol volume fraction of80%,and the extraction time of 4 h.Under above conditions,the extraction rate of total flavonoids reaches5.33%.The method possesses the characteristics of high precision,stability,and reproducibility,and the recovery is 97.213%.
作者
雒江菡
LUO Jiang-han(Institute of Cell and Molecular Biology,College of Pharmacy,Harbin University of Commerce,Harbin 150001,China)
出处
《化学与生物工程》
CAS
2018年第8期50-52,62,共4页
Chemistry & Bioengineering
关键词
百蕊草
总黄酮
提取工艺
优化
正交实验
Thesium chinense
total flavonoids
extraction process
optimization
orthogonal experiment
作者简介
雒江菡（1980），女，山东宁津人，博士，讲师，研究方向：中药学，Email：649993192@qq．com。
