摘要
目的 :探讨铁超载与铁剥夺对依托泊甙 (VP 1 6)诱导HL 60细胞凋亡的影响。方法 :选择 1 0 0 μmol/LFeCl3和 1 0 μmol/L去铁胺 (DFO)与VP 1 6共同作用于HL 60细胞 ,并以单用VP 1 6作对照。光镜观察HL 60细胞形态学变化、DNA琼脂糖电泳及流式细胞仪 (FCM )等方法检测HL 60细胞凋亡。观察铁超载与铁剥夺对VP 1 6诱导HL 60细胞凋亡的影响。结果 :①FeCl3(1 0 0 μmol/L) +VP 1 6 (1 0 0mg/L) 6h ,1 2h ,2 4h ,细胞凋亡率(APO % )和亚二倍体峰 (Sub G1 ) ,低于单用VP 1 6(1 0 0mg/L)组 ,2组相比差异有统计学意义 (P <0 .0 5)。DNA电泳显示ladder出现时间滞后、数目减少。②DFO(1 0 μmol/L) +VP 1 6 (1 0 0mg/L )组APO %、DNA电泳ladder数目、Sub G1均比单用VP 1 6高 (P <0 .0 1 )。③等量的DFO与FeCl3和VP 1 6(1 0 0mg/L)与单用VP 1 6(1 0 0mg/L)结果相同。结论 :铁超载抑制化疗药物VP 1 6诱导HL 60细胞凋亡作用 ,铁剥夺可促进VP 1 6诱导HL 60细胞凋亡作用。临床上可采用铁剥夺的方法协同治疗白血病 。
Aim: To explore the effects of iron overload and iron deprivation on apoptosis of HL 60 cells induced by VP 16.Methods: The cell culture,cell morphology, flow cytometry assay,and DNA gel electrophoresis were used to detect the apoptosis of HL 60 cells.Choosing Ferric(III) chloride (FeCl 3) (100 μmol/L), iron chelator desferrioxamine(DFO) (10 μmol/L),and etoposide (VP16) (1 mg/L,10 mg/L,and 100 mg/L) induced the apoptosis of HL 60 cells, choosing normal saline and VP16 as control.Results: ①When HL 60 cells cultured with 100 μmol/L FeCl 3+100 mg /L VP 16 for 6 h,12 h,and 24 h, APO% and Sub G1% were lower than that of VP 16 group ( P < 0.05 ). DNA electrophoresis showed that the number of ladder decreased and the present time of ladder lagged. ②Compared 10 μmol/L DFO + 100mg/ L VP 16 group with VP 16 group, APO%, the number of the ladders, and Sub G1% of the former were much higher than that of the latter.③Equimolar concentration of FeCl 3 and DFO + VP 16, APO% the number of the ladders, and Sub G1% had no significant difference compared with VP 16. Conclusions: Iron overload could inhibit apoptosis of HL 60 cells induced by VP 16,while iron deprivation may promote apoptosis of HL 60 cells induced by VP 16. So iron deprivation could be used as an assistant in treating leukemia and supplement of iron for patients with leukemia should be catious.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2002年第5期591-593,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省自然科学基金资助项目 984 0 2 150 0
