摘要
目的建立采用超声提取,高效液相色谱法(HPLC)测定山葡萄藤中白藜芦醇的含量。方法采用色谱柱:Inertsil ODS-3 C18色谱柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.05%磷酸溶液(40:60);流速:1.0 mg/m L;柱温:30℃;检测波长:306 nm;进样量:10μL。结果白藜芦醇在0.504~75.6 mg/m L范围内呈良好的线性关系。测定10批山葡萄藤中白藜芦醇平均含量为0.48%,平均回收率95.16%(RSD=1.94%,n=9)。结论该实验采用超声提取,并对提取条件进行探讨,得出最佳条件为超声时间45 min、超声功率300 W、固液比1∶250(g/m L)、溶剂为甲醇。该研究表明该方法简便、快速、经济、可靠,专属性强,可用于山葡萄藤的质量控制。
Objective Ultrasonic extraction and high performance liquid chromatography（HPLC） were used to determine the content of resveratrol in grape vines. Methods Chromatography column： inertsil ODS-3 C18 column（250 mm×4.6 mm,5 μm）;mobile phase： methanol-0.05% phosphoric acid solution（40：60）; flow rate： 1.0 m L/min; column temperature ： 30°C; detection wavelength： 306 nm; injection volume： 10 μL. Results Resveratrol showed a good linear relationship in the range of0.504 to 75.6 mg/m L. The average content of resveratrol in 10 batches of vines was 0.48%, and the average recovery was95.16%（RSD=1.94%,n=9）. Conclusion Ultrasonic extraction was used in this experiment and the extraction conditions were investigated. The optimal conditions were： ultrasonic time 45 min, ultrasonic power 300 W, solid-liquid ratio 1：250（g/m L）,and methanol as the solvent. The study shows that this method is simple, rapid, economical, reliable, and highly specific. It can be used for quality control of vine vines.
作者
杨荣艳
刘琬晖
高楠楠
YANG Rong-yan, LIU Wan-hui, GAO Nan-nan(Siping Food and Drug Inspection Institute, Siping, Jilin Province, 136000 China)
出处
《中国卫生产业》
2018年第15期6-8,共3页
China Health Industry
基金
吉林省中药材标准化项目(2016009)
关键词
山葡萄藤
高效液相色谱法
白藜芦醇
Mountain vines
High performance liquid chromatography
Resveratrol
作者简介
杨荣艳（1978-），女，吉林四平人，硕士，副主任药师，主要从事中药检验工作。
