摘要
为从发酵液中分离纯化D-3-苯基乳酸(D-3-phenyl lactic acid,PLA),利用重组大肠杆菌E.coli p ET-28aldhY52V转化底物苯丙酮酸(phenylpyruvic acid,PPA)合成PLA,采用离子交换法对PLA纯化工艺进行了初步研究。通过对6种常用阴离子交换树脂对PLA的静态吸附容量测定,确定了D315具有较强的PLA吸附性能。在固定床上样条件优化结果表明,上样流速为4 BV/h、径高比为1∶7时,上样吸附效果较好。利用二阶段洗脱工艺:第一阶段用100 mmol/L HCl进行洗脱,第二阶段用200 mmol/L Na Cl在5 BV/h流速下洗脱。洗脱验证结果表明,第一阶段洗脱液中PLA/PPA的质量浓度比值由初始的4.2上升到10.3,PLA收率为66.4%。研究结果为进一步采用离子交换法纯化发酵液中PLA的工艺优化提供了借鉴。
In order to purify D-3-phenyl lactic acid（ PLA） from fermentation broth,the purification process of PLA by ion-exchange method was studied after converting phenylpyruvic acid（ PPA） into PLA by using recombinant E. coli p ET-28 a-ldhY52 V. The D315 was selected from six kinds of anion exchange resins for its better static adsorption capacity of PLA. The optimal absorption efficiency was achieved when the sample flow rate was 4 BV/h and the ratio of diameter to height was 1∶ 7. A two-stepwise elution process was found to separate PLA from PPA effectively. In the first step,100 mmol/L HCl was used as eluent,and in the second step,200 mmol/L Na Cl was used as eluent and the flow rate was 5 BV/h. The results of verification experiment showed that yield of PLA reached 66. 4% in the first step and the mass ratio of PLA to PPA was increased from 4. 2 in the fermentation broth to 10. 3. The report provided a reference for further optimizing the PLA purification process using anion exchange method.
作者
郑青云
程俊
崔春霞
朱益波
ZHENG Qing-yun;CHENG Jun;CUI Chun-xia;ZHU Yi-bo(Changshu Institute of Technology,Changshu,215500,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2018年第6期119-125,共7页
Food and Fermentation Industries
基金
国家自然科学基金青年科学基金项目(31501459)
常熟市社会发展项目(CR201709)
作者简介
本科.;朱益波副教授为通讯作者,E-mail：centuryrain@126.com.
