摘要
[目的]本研究明确鼓槌石斛花朵挥发性成分及其释放量变化,阐明其花香气形成的物质基础,为石斛属植物花香研究提供理论基础。[方法]以鼓槌石斛原生种为材料,运用固相微萃取(SPME)结合GC-MS技术测定鼓槌石斛不同花期及盛花期时一天中不同时段花朵挥发性成分及其释放量变化。[结果]从鼓槌石斛花朵释放的花香中共鉴定出花香成分33种,其中,萜烯类15种,酯类7种,醇类6种,芳香族化合物2种,酮类2种,醛类1种;鼓槌石斛花香成分主要为酯类和萜烯类,其释放量占总香气成分的80%以上。鼓槌石斛盛花期花朵随一天中开放时段的不同,香气成分种类和释放量皆呈先上升后下降的趋势,其中,香气种类和释放量均在14:00时达到最高。鼓槌石斛不同花期挥发性成分中的酯类、萜烯类、醛类、醇类、酮类和芳香族化合物的种类和释放量皆呈先上升后下降的趋势,在盛花期达到最高。[结论]本研究明确鼓槌石斛花朵不同花期及盛花期一天中的不同时段挥发性成分及释放量差异明显,乙酸辛酯、β-罗勒烯、α-蒎烯和苯乙醛是影响鼓槌石斛香气的主要物质,为探讨石斛兰香气形成机制和香花型石斛兰育种提供参考。
[ Objective ] To analyze the aroma compounds in flowers of Dendrobium chrysotoxum, in order to clarify the material basis of floral scents fomlation and provide reference for bleeding. [ Method ] The aromatic component categories and their release amounts in flowers of native Den. chrysotoxum in different florescence stages were detec- ted and the diurnal variation of full blooming stage were determined by solid-phase microextraction （SPEM） and gas chromatography coupled with mass spectrometry （GC-MS）. [ Result] The floral scents of 33 volatiles were identi- fied as scent components in Den. chrysotoxum, including 15 terpenes, 7 esters, 6 alcohols, 2 aromatic compounds, 2 ketones and 1 aldehydes. Ester and terpenoids were the dominant components of these scents, composing exceed 80% to all detected compounds. The aromatic component categories and their release amounts at full blossoming stage increased at first and then decreased at different sampling time, and reached the maximum at 2：00 p.m. In different blooming stages, the contents of terpenes, esters, alcohols, aromatic compounds, aldehydes and ketones increase and then decrease, and reached the highest and their release amounts are distinctly different at d Den. chrysotoxum stances responsible for the floral Octyl acetate, scent formation β-ocimene opening stage. [ Conclusion ] The aroma florescence stages and show a diurnal varia , α-pinene and benzeneacetaldehydo are the hrysotoxum. compounds tion in full main sub-stances responsible for the floral scent fomlation in Den. chrysotoxum.
作者
黄昕蕾
郑宝强
王雁
HUANG Xin-lei;ZHENG Bao-qiang;WANG Yah(Research Institute of Forestry,Chinese Academy of Forestr;Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Beijing 100091,Chin)
出处
《林业科学研究》
CSCD
北大核心
2018年第4期142-149,共8页
Forest Research
基金
国家科技支撑计划(2013BAD0180703)
关键词
鼓槌石斛
花期
日变化
花香
GC/MS技术
Dendrobium chrysotoxum
florescence
diurnal variation
aroma component
GC/MS
作者简介
黄听蕾（1985-），女．博士，主要研究方向为花卉遗传改良．E—mail：360449284@qq．com;通讯作者：王雁（1969-），女，博士，研究员．E—mail：chwy8915@sina．com
