摘要
目的探讨小檗碱对高糖诱导肾小球足细胞损伤的保护作用及其机制。方法体外培养小鼠肾小球足细胞,分为正常对照组、高糖模型组、不同浓度小檗碱组(30、60、90μmol·L^(-1)),分别处理足细胞。MTT法检测小檗碱对高糖诱导足细胞增殖能力的影响;Annexin V-FITC/PI双染实验观察小檗碱对高糖诱导足细胞凋亡的影响;利用Transwell小室观察小檗碱对高糖诱导足细胞迁移能力的影响;Western blot法检测小檗碱对高糖诱导足细胞nephrin、podocin、desmin蛋白表达的影响;黏附能力实验检测小檗碱对高糖诱导足细胞黏附功能的影响。结果 MTT检测结果显示,小檗碱能增加高糖诱导足细胞的活力;Annexin V-FITC/PI双染实验结果显示,小檗碱能抑制高糖诱导足细胞的凋亡;Transwell迁移实验结果显示,小檗碱可以抑制高糖诱导足细胞的迁移;Western blot结果显示,小檗碱可降低高糖诱导足细胞desmin蛋白表达,明显升高nephrin与podocin蛋白的表达;黏附功能实验结果表明,小檗碱能明显提高高糖诱导足细胞的黏附能力。结论小檗碱改善高糖诱导足细胞功能的损伤,其作用机制可能与保护足细胞nephrin和podocin蛋白相关。
Aim To investigate the protective effect of berberine on high glucose（ HG） induced podocytes injury and the detailed mechanism. Methods Mouse glomerular podocytes were cultured in vitro and divided into control group, HG model group, and different concentrations of berberine（ 30,60,90 μmol·L-1）treated group respectively. The cell proliferation was detected by MTT assay; the apoptosis was detected by Annexin V-FITC/PI double staining; the ability of migration was observed by transwell assay; the expressions of nephrin,podocin and desmin were detected by Western blot; the adhesion function was detected by adhesion ability assay. Results The HG-induced podocyte viability was significantly promoted by berberine（ 30,60,90 μmol·L-1）. The apoptosis and migration of HG-induced podocyte significantly decreased after exposure to berberine. After treatment with berberine（ 30,60,90 μmol·L-1） in HG-induced podocyte,the expression of nephrin and podocin markedly increased,while desmin expression significantly decreased. Meanwhile,the adhesion ability increased after treated with berberine. Conclusion Berberine improves the damage of podocyte induced by HG,which may be related to the protection of nephrin and podocin in podocytes.
作者
王盈盈
刘青
唐丽琴
魏伟
WANG Ying-ying;LIU Qing;TANG Li-qin;WEI Wei(Institute of Clinical Pharmacology,Anhui Medical University,Key Lab of Antiinflammatory and Immune Medicine,Ministry of Education,Collaborative Innovation Center of Antiinflammatory and Immune Medicines,Hefei 230032,China;Dept of Pharmacy,Anhui Provincial Hospital,Anhui Medical University,Hefei 230001,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2018年第8期1158-1163,共6页
Chinese Pharmacological Bulletin
作者简介
王盈盈（1993-）,女,硕士生,研究方向：内分泌及代谢药理学,E-mail：wangyingying601＠163.com;;唐丽琴（1972-）,女,博士,主任药师,教授,硕士生导师,研究方向：内分泌及代谢药理学,通讯作者,E-mail：tulcyl＠sina.com;;魏伟（1960-）,男,博士,教授,博士生导师,研究方向：抗炎免疫药理学,通讯作者,E-mail：wwei＠ahmu.edu.cn
