摘要
为探讨不同温度条件下南方根结线虫对不同抗性烟草品种生理生化指标的影响,利用光合培养箱控制烟草生长环境的温度,采用人工接种南方根结线虫的方法,对不同温度条件下各时期烟草品种根系生理生化指标进行测量。研究表明,受到南方根结线虫侵染后,不同抗性烟草品种根系过氧化物酶(POD)、过氧化氢酶(CAT)活性及羟脯氨酸糖蛋白、可溶性糖、脯氨酸含量均增加,但超氧化物歧化酶(SOD)活性降低,当温度为25℃时,各生理生化指标变化幅度最大,温度为35℃时变化最小。因此,35℃高温对南方根结线虫侵染活动影响最大,SOD、POD、CAT活性及羟脯氨酸糖蛋白、可溶性糖、脯氨酸含量与烟草抗南方根结线虫有密切的关系。
In order to investigate the effects ofMeloidogyn~ incognita （M.incognita） on physiological and biochemical indexes of resistant and susceptible tobaccos （Nicotiana tabacum） under different temperatures, the indexes of roots at each stage were determined. The growing temperatures were controlled by photosynthetic incubator and the M. incognita inoculated artificially. According to the results, Peroxidase （POD）, Catalase （CAT） activities, hydroxyproline glycoprotein （HRGP）, soluble sugar and proline contents were significantly increased in all varieties, while Superoxide dismutase （SOD） activity decreased. The range of physiological and biochemical indexes reached maximum at 25 ℃ while minimum at 35 ℃. Therefore, at higher temperature such as 35 ℃ might have the greatest influence on M.incognita invasion. SOD, POD, CAT activities, hydroxyproline glycoprotein, soluble sugar and proline contents may closely relate to the tobacco resistance to M.incognita.
作者
王袁
郭泽
李晓辉
徐世晓
邢学霞
张思琦
何佳
刘超
陈芳
杨铁钊
Wang Yuan;Guo Ze;Li Xiaohui;Xu Shixiao;Xing Xuexia;Zhang Siqi;He Jia;Liu Chao;Chen Fang;Yang Tiezhao(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450002,Henan,Chin)
出处
《作物杂志》
CAS
北大核心
2018年第4期161-166,共6页
Crops
基金
中国烟草总公司重大项目(110201402004)
关键词
南方根结线虫
温度
烟草根系
生理特性
M.incognita
Temperature
Tobacco root system
Physiological characteristics
作者简介
王袁，硕士研究生，主要从事烟草品种对南方根结线虫抗性的研究;杨铁钊为通信作者，教授，从事烟草遗传育种与品质改良研究
