摘要
采用HPLC-UV/MS检测方法研究了链霉菌Streptomyces sp.CSDX076菌株次级代谢产物中新天然产物A的发酵条件优化.对碳源、氮源组合不同的培养基在不同发酵时间、萃取溶剂不同的条件下得到的新天然产物A进行了定性及半定量的研究.结果发现,在基础培养基中加入8g/L用双蒸水浸泡的腐殖酸,培养13 d,乙酸乙酯萃取得到的天然产物A的生物量最高.
HPLC-UV/MS detection method was adopted to study the optimal fermentation condition of the new natural product A in the secondary metabolites of Streptomyces sp. CSDX076. Both qualitative and semi-quantitative studies of the new natural product A were carried out with different fermentation times and extraction solvents as well as different ingredients of the carbon sources and nitrogen sources. 8 g/L of humic acid soaked in the double steaming water for 24 h was added to the base medium. The highest biomass of the natural product A was in 13 days,and the extraction solvent was ethyl acetate.
作者
钱声艳
李彩玉
周兆通
杨彩玲
李爱苹
N Sheng-yan;LI Cai-yu;ZHOU Zhao-tong;YANG Cai-ling;LI Ai-ping(Guizhou Key Laboratory of Microbial Research ＆ Drug Development,Zunyi Medical University,Zunyi 563000,China;Guizhou Key Laboratory of Stomatological Research,Zunyi Medical University,Zunyi 563000,China;Yunnan Institute of Food Safety,Kunming University of Science and Technology,Kunming,650500,China;College of Pharmacy,Zunyi Medical University,Zunyi 563000,China)
出处
《云南民族大学学报（自然科学版）》
CAS
2018年第4期270-273,293,共5页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
遵义医学院硕士启动基金(F-757)
遵义科技局市校联合基金(E-215)
贵州省普通高等学校微生物资源与药物开发重点试验开放课题(GZMRD-2015-001)
作者简介
钱声艳（1984-），女,硕士,实验师.主要研究方向：微生物天然产物发现与活性研究.
