摘要
褐飞虱Nilaparvata lugens(Stl)是水稻最主要的害虫,其繁殖力高低是影响水稻产量损失最直接的因素。本文旨在研究褐飞虱卵巢特异性基因Nl10847对褐飞虱繁殖力的影响。首先通过克隆验证了Nl10847的开放阅读框(ORF)为552 bp,编码184个氨基酸;然后利用q-PCR技术分析其时空表达模式,发现其在卵巢组织和成虫期高表达;最后通过RNAi的方法,发现下调Nl10847基因的表达后会抑制全虫和卵巢中NlVgR的mRNA表达水平,并严重阻碍卵巢的发育。这些结果表明Nl10847在卵巢发育成熟过程中承担重要的功能。
The brown planthopper（BPH） Nilaparvata lugens（Stl） is one of the most serious insect pests of rice. Its fecundity is the direct factor determining the extent of damage to rice. This paper aims to study the effects of ovarian specific gene Nl10847 on the fecundity of BPH. Firstly,the open reading frame（ORF） of Nl10847 was cloned and verified to be 552 bp, encoding 184 amino acids.Subsequently,q-PCR was used to analyze the spatiotemporal expression patterns,and high expression was found in ovarian tissue and adult stage. Finally,by using RNAi,it was found that the downregulated expression of Nl10847 inhibited the mRNA expression level of NlVgR in the whole insect and the ovary,and seriously delay ovary development of the insects. These results suggest that Nl10847 play an important role in ovarian development and maturation.
作者
黎丹
官展文
黄诗韵
张文庆
LI Dan;GUAN Zhan-Wen;HUANG Shi-Yun;ZHANG Wen-Qing(State Key Laboratory of Biocontrol and School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)
出处
《环境昆虫学报》
CSCD
北大核心
2018年第3期497-504,共8页
Journal of Environmental Entomology
基金
国家自然科学基金项目(31672021)
作者简介
黎丹,女,1993年生,湖南耒阳人,硕士生,研究方向为农业昆虫与害虫防治,E-mail：Iidan88@mail2.syu.edu.cn;通讯作者Author for correspondence,E-mail：lsszzq@mail.sysu.edu.cn
