摘要
该研究以实验室构建的重组大肠杆菌E.coli BL21/p ET28a(+)-mle为研究对象,以L-乳酸量为评价指标,通过单因素和正交试验研究了诱导温度、培养基起始pH值、异丙基-β-D-硫代吡喃半乳糖苷(IPTG)浓度和诱导时间对重组大肠杆菌E.coli BL21/p ET28a(+)-mle分泌表达苹果酸乳酸酶(MLE)的影响。结果表明,重组大肠杆菌E.coli BL21/p ET28a(+)-mle分泌表达苹乳酶的最佳培养条件为培养基起始pH值为5.9,诱导温度为28℃,IPTG浓度0.6 mmol/L,诱导时间3 h。在此优化条件下,L-乳酸产量为2.37 g/L,较优化前提高9.48倍。
Using the constructed recombinant E. coli BL21/p ET28 a（＋）-mle as research object, and L-lactic acid production as evaluation index, the effects of inducing temperature, medium initial pH, isopropyl-β-D-1-thiogalactopyranoside（IPTG） concentration, and inducing time on malolactic enzyme production secreted and expressed by E.coli BL21/p ET28 a（＋）-mle were studied using single factor and orthogonal experiments. The result showed that the optimal culture conditions for the secretory and expression of malolactic enzyme by recombinant E. coli BL21/p ET28 a（＋）-mle were：initial medium pH 5.9, inducing temperature 28 ℃, IPTG concentration 0.6 mmol/L, and inducing time 3 h. Under the optimized condition, the yield of L-lactic acid was 2.37 g/L, which was 9.48 times higher than that of before optimization.
作者
胡兰兰
田亚楠
陈福生
张秀艳
HU Lanlan;TIAN Yanan;CHEN Fusheng;ZHANG Xiuyan(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,Chin)
出处
《中国酿造》
CAS
北大核心
2018年第6期161-164,共4页
China Brewing
基金
宁夏自治区重点研发计划重大项目(2016BZ06-03)
关键词
大肠杆菌
苹果酸乳酸酶
发酵条件
优化
L-乳酸
Escherichia coli
malolactic enzyme
fermentation conditions
optimization
L-lactic acid
作者简介
胡兰兰（1993-），女，硕士研究生，研究方向为食品微生物。;通讯作者：张秀艳（1973-），女，副教授，博士，研究方向为食品生物技术与安全。
