摘要
In this research,an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid.The efficacy of the air plasma on bacterial deactivation and the cytobiological variations after the plasma treatment are investigated.According to colony forming units,nearly all the bacteria（6-log） are inactivated after 10 min of air plasma treatment.However,7% of the bacteria enter a viable but non-culturable state detected by the resazurin based assay during the same period of plasma exposure.Meanwhile,86% of the bacteria lose their membrane integrity in the light of SYTO 9/PI staining assay.The morphological changes in the cells are examined by scanning electron microscopy and bacteria with morphological changes are rare after plasma exposure in the liquid.The concentrations of the long-living RS,such as H2O2,NO3^- and O3,in liquid induced by plasma treatment are measured,and they increase with plasma treatment time.The changes of the intracellular ROS may be related to cell death,which may be attributed to oxidative stress and other damage effects induced by RS plasma generated in liquid.The rapid and effective bacteria inactivation may stem from the RS in the liquid generated by plasma and air plasmas may become a valuable therapy in the treatment of infected wounds.
In this research,an atmospheric-pressure air plasma is used to inactivate the multidrug-resistant Acinetobacter baumannii in liquid.The efficacy of the air plasma on bacterial deactivation and the cytobiological variations after the plasma treatment are investigated.According to colony forming units,nearly all the bacteria（6-log） are inactivated after 10 min of air plasma treatment.However,7% of the bacteria enter a viable but non-culturable state detected by the resazurin based assay during the same period of plasma exposure.Meanwhile,86% of the bacteria lose their membrane integrity in the light of SYTO 9/PI staining assay.The morphological changes in the cells are examined by scanning electron microscopy and bacteria with morphological changes are rare after plasma exposure in the liquid.The concentrations of the long-living RS,such as H2O2,NO3^- and O3,in liquid induced by plasma treatment are measured,and they increase with plasma treatment time.The changes of the intracellular ROS may be related to cell death,which may be attributed to oxidative stress and other damage effects induced by RS plasma generated in liquid.The rapid and effective bacteria inactivation may stem from the RS in the liquid generated by plasma and air plasmas may become a valuable therapy in the treatment of infected wounds.
作者
Zhe RUAN
Yajun GUO
Jing GAO
Chunjun YANG
Yan LAN
Jie SHEN
Zimu XU
Cheng CHENG
Xinghao LIU
Shumei ZHANG
Wenhui DU
Paul K CHU
阮哲;郭亚俊;高静;杨春俊;兰彦;沈洁;许子牧;程诚;刘行浩;张书梅;杜文辉;朱剑豪(Department of Dermatology,the Second Affiliated Hospital,Anhui Medical University;Institute of Plasma Physics,Chinese Academy of Sciences;School of Resources and Environmental Engineering,Hefei University of Technology;Department of Dermatology,the First Affiliated Hospital,Anhui Medical University;Department of Physics and Department of Materials Science and Engineering,City University of Hong Kong)
基金
supported by the Spark Program of the second Affiliated Hospital of Anhui Medical University (Grant No.2015hhjh04)
National Natural Science Foundation of China under Grant No.51777206
Natural Science Foundation of Anhui Province (Grant No.1708085MA13 and No.1708085MB47)
Science Foundation of Institute of Plasma Physics,Chinese Academy of Sciences under Grant (No.DSJJ-14-YY02)
City University of Hong Kong Applied Research Grant (ARG) (No.9667144)
Hong Kong Research Grants Council (RGC) General Research Funds (GRF) (No.City U 11301215)
Doctoral Fund of Ministry of Education of China (No.2017M612058)
Specialized Research Fund for the Doctoral Program of Hefei University of Technology (No.JZ2016HGBZ0768)
Foundation of Anhui Province Key Laboratory of Medical Physics and Technology (Grant No.LMPT2017Y7BP0U1581)
作者简介
E-mail： yangchunjun9@163.com;chengcheng@ipp.ac.cn and paul.chu@cityu.edu.hk
