摘要
目的:研究含与不含佐太的安置精华散对功能性消化不良(FD)肝郁脾虚型大鼠胃粘膜屏障损伤和紧密连接蛋白表达的影响,探讨佐太在复方中的作用。方法:将Wistar大鼠随机分组,空白组正常饲养,模型组、安置精华散组和当佐组(含佐太的安置精华散组),按照复合病因造模法造成FD肝郁脾虚模型;造模28天后,模型组继续造模,安置精华散和当佐组造模同时灌胃给予相应药物,连续14天。HE染色观察胃体组织病理变化,RT-PCR检测紧密连接相关基因表达;Western-blot检测凋亡相关蛋白表达。结果:与空白组相比,模型组大鼠胃排空实验胃内残留率显著升高,小肠推进率显著降低,胃黏膜细胞排列紊乱,腺体间隙扩大;胃上皮细胞紧密连接蛋白OCLN mRNA的表达显著下降,粘附分子ICAM-1 mRNA的表达显著增高;胃体组织凋亡相关蛋白Caspase-3和Caspase-8的表达显著增加;与模型组相比,50.75mg/kg安置精华散和52.48mg/kg当佐可以从不同程度逆转上述结果,两者之间未见显著差异。结论:含与不含佐太的安置精华散对FD大鼠胃黏膜损伤具有一定的保护作用,主要是通过下调胃窦区凋亡因子的表达,上调紧密连接蛋白的表达,减轻胃黏膜屏障损伤;佐太在复方中的作用有待于进一步研究。
Objective： To explore the possible contribution of Zuotai to pharmacodynamic activies of AZ by comparing effects of Anzhi Jinghua San（AZ） with and without Zuotai on gastric mucosa barrier injury and the expression of tight junction protein in type Ganyu Pixu of gastric tissues of rats with functional dyspepsia（FD）. Methods： All rats were randomly divided into the control group,the model group,AZ group and Dangzuo（DZ） group. Except the control group,rats in other groups were made FD model for 42 days. After 28 days of modeling,AZ was given to rats in AZ group and DZ was given in DZ group. Gastrointestinal motility,gastric mucosa damage and the expression of tight junction protein were observed. Results： Compared with the control group,AZ and DZ improved the function of gastric emptying,promoted intestinal propulsion,lessened gastric mucous injury,increased the mRNA expression of tight junction protein and decreased protein expressions of Caspase-3 and Caspase-8. Conclusion： AZ and DZ could obviously improve FD symptoms in rats,which was associated with the improvement of gastric tight junction protein. Further research is needed to confirm the role of Zuotai in AZ.
作者
陈海娟
李岑
夏振江
魏立新
尚靖
Chen Haijuan;Li Cen;He Qiangqiang;Wei Lixin;Shang Jing(University of Chinese Academy of Sciences,Beijing 10049;Key Laboratory of Tibetan Medicine Research,Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008;State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009;Qinghai Normal University School of Life Sciences,Xining 810003)
出处
《中药药理与临床》
CAS
CSCD
北大核心
2018年第3期133-136,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
中国科学院百人计划(Y229461211)
青海省应用基础研究项目(2015-ZJ-733)
青海省自然科学基金(2016-ZJ-919)
青海省重点实验室发展专项基金(2014-Z-Y02)
关键词
安置精华散
当佐
佐太
肝郁脾虚
胃粘膜
紧密连接蛋白
Anzhi Jinghua San
Dangzuo
Zuotai
Gastric mucosa
Tight junction protein
Type Ganyu Pixu
作者简介
通讯作者:尚靖
