摘要
目的:观察不同浓度密蒙花滴眼液对去势雄兔泪腺细胞炎症因子TNF-α、IL-1β的影响,探讨不同浓度密蒙花滴眼液对去势雄兔干眼症模型的疗效。方法:将密蒙花原药材制备成低、中、高3种浓度滴眼液。将42只健康成年新西兰长耳白兔(雄性),随机分为A:空白组;B:模型组;C:低浓度密蒙花滴眼液组;D:中浓度密蒙花滴眼液组;E:高浓度密蒙花滴眼液组;F:安慰剂滴眼液组;G:睾酮组等7组,每组6只。除A组外其余6组实验用兔行双侧睾丸及附睾切除术。从术后第3天开始C、D、E、F组分别予相应滴眼液滴双眼。各组实验用兔,分别于术前和术后第4wk各进行一次Schirmer I试验(SIT)和泪膜破裂时间(BUT)测定。采用免疫组化法对泪腺细胞炎症因子TNF-α和IL-1β的表达进行检测。结果:1)SIT与BUT值比较:术后C组与D、E、G组比较,有统计学意义(P<0.01);D、E、G组间相比较,无统计学意义(P>0.05);2)术后各组炎症因子TNF-α、IL-1β比较:C组与D、E、G组比较,有统计学意义(P<0.05);D、E、G组间相比较,无统计学意义(P>0.05)。结论:1)密蒙花滴眼液具有与雄激素相似的抑制细胞炎症因子表达的作用,但其作用弱于雄激素;2)中、高浓度密蒙花滴眼液对TNF-α和IL-1β的抑制作用要强于低浓度密蒙花滴眼液,但中、高浓度密蒙花滴眼液的抑制作用差别不明显。
AIM： To observe the effect of different concentrations Buddleja offcinalis eye drops（ BOED） to inflammatory factors of lacrimal gland cells of castrated male rabbit with dry eye. METHODS： Forty-two healthy adult New Zealand rabbits were randomly divided into 7 groups,6 rabbits each group. Group A： blank group; Group B： model group; Group C： low concentrations BOED group; Group D： medium concentrations BOED; Group E： high concentrations BOED; Group F： the placebo group;Group G： testosterone groups. All rabbits of groups B to G were cut off bilateral testis and epididymis except group A. The rabbits of groups C,D,E and F were dropped relevant eye drops except groups A and B. All rabbits were tested for Schimer I test（ SIT） and break-up time（ BUT） before operation and 4 wk after operation.Expressions of inflammatory factors of TNF-α,IL-1β from lacrimal gland cells were checked in immunohistochemical staining method.RESULTS： 1） Comparison of SIT and BUT： Compared with the groups D,E and G, group C had statistical significance（ P〈0. 01）. Compared with groups D and E,G groups,there was no significant difference among those group after operation（ P〈0. 05）; 2） Comparison of inflammatory factors of TNF-α,IL-1β after operation：Compared with value of the average optical density of TNF-α and IL-1β of group C,those of groups D,E and G were obviously different after operation（ P〈0. 05）.Compared D with E,G groups,there was no significant difference between those group after operation（ P〈0. 05）.CONCLUSION： 1） Compared with androgen,BOED has the similar effect of depressing the expression of inflammatory factors. But its effect is slightly weaker than the androgen; 2） The depressing effect of medium and high concentration BOED are better than low concentration. Compared with medium and high concentration of BOED, there was no significant difference between two groups.
作者
彭俊
欧阳云
李文娟
吴权龙
谭涵宇
彭清华
Jun Peng;Yun Ouyang;Wen-Juan Li;Quan-Long Wu;Han-Yu Tan;Qing-Hua Peng(Key Discipline of Ophthalmology of Chinese Medicine in Hunan University of Chinese Medicine,Changsha 410208 Hunan Province,China)
出处
《国际眼科杂志》
CAS
北大核心
2018年第8期1359-1364,共6页
International Eye Science
基金
国家自然科学基金面上资助项目(No.81574031)
湖南省高层次卫生人才“225”工程培养项目资助
湖南省科技厅科研基金资助项目(No.2015SF2016-6)
湖南省发展改革委科研基金资助项目(湘发改投资[2014]658号)
中医药防治眼耳鼻咽喉疾病湖南省重点实验室建设项目(No.2017TP1018)
长沙市科技计划项目(No.KC1704005).
关键词
密蒙花滴眼液
干眼
去势雄兔
泪腺细胞
炎症因子
Buddleja offcinalis eye drops
dry eye
lacrimal gland cell
inflammatory factors
作者简介
彭俊,湖南中医药大学,硕士.研究方向：眼表疾病、青光眼及眼底病的中医药研究.;通讯作者：谭涵宇,湖南中医药大学,博士,讲师.研究方向：眼表疾病、青光眼及眼底病的中医药研究.t%612@126.com;通讯作者：彭清华,湖南中医药大学,博士,教授.研究方向：眼表疾病、青光眼及眼底病的中医药研究.pqh410007@126.com;
