摘要
以箬叶黄酮碳苷母核木犀草素为虚拟模板分子,结合Zn（Ⅱ）的配位作用,丙烯酰胺为功能单体,N,N＇-亚甲基双丙烯酰胺（MBA）为交联剂,乙醇为溶剂制备了木犀草素-Zn（Ⅱ）配位印迹聚合物,并将此聚合物与固相萃取相结合,分离了醇水体系中的箬叶黄酮碳苷。结果表明：当n（模板分子）∶n（功能单体）∶n（交联剂）=1∶5∶30时,配位印迹聚合物具有最好的吸附性能,对醇相中木犀草素的吸附容量可达103.40 mg/g,是未配位印迹聚合物吸附容量（60.61 mg/g）的1.71倍,空白印迹聚合物（7.33 mg/g）的14.11倍。采用木犀草素-Zn（Ⅱ）配位印迹聚合物分离醇水相中箬叶黄酮碳苷,经一次分离后,荭草苷、异荭草苷、牡荆苷和异牡荆苷的回收率分别为91.95%、80.87%、60.43%和63.63%;质量分数分别是粗提物的55.36、48.89、36.64和38.35倍,4种黄酮碳苷总质量百分数由提取前的0.70%提高到32.68%。
A series of luteolin-Zn（Ⅱ） complex molecularly imprinted polymers（MIPs） were synthesized in ethanol, using luteolin（the mother nucleus of C-glycosyflavones from Indicalamus leaf） as dummy template, acrylamide（AM） as monomer and N, N＇-methylene diacrylamide（MBA） as cross-linker. And then C-glycosyflavones from Indicalamus leaf in alcohol-water system were separated by solid phase extraction using MIPs. The results showed that when n（luteolin-Zn）∶n（AM）∶n（MBA） was 1∶5∶30, the obtained complex molecularly imprinted polymer possessed the highest adsorption properties, and the adsorption capacity for luteolin from ethanol phase reached 103.40 mg·g–1, which was 1.71 times that of uncoordinated molecularly imprinted polymer（60.61 mg/g）, 14.11 times that of blank polymer（7.33 mg/g）. After a separation, the recovery rates of orientin, isoorientin, vitexin and isovitexin were 91.95%, 80.87%, 60.43% and 63.63%, compared with crude extract, the corresponding mass percents were increased by 55.36, 48.89, 36.64 and 38.35 times, respectively. In addition, the total mass percent of four C-glycosyflavones was increased to 32.68% from 0.70% before extraction.
作者
乐薇
吉瑞冬
吴士筠
YUE Wei;JI Rui-dong;WU Shi-jun(College of Environmental and Biological Engineering, Wuhan Technology and Business University, Wuhan 430065, Hubei, Chin)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2018年第7期1156-1162,共7页
Fine Chemicals
基金
湖北省教育厅科学研究计划项目(B2017321)
武汉工商学院校学术团队资助项目(SXTD2015003)~~
关键词
木犀草素
配位分子印迹聚合物
箬叶
黄酮碳苷
中药现代化技术
luteolin
complex molecularly imprinted polymer
Indicalamus leaf
C-glycosyflavones
modernization technology of traditional Chinese medicines
作者简介
乐薇（1979-），女，副教授，E—mail：yuewei11@126．com
