摘要
【目的】观察感染后肠易激综合征小鼠肠组织中IL-17、occludin和ZO-1的动态改变。【方法】40只C57BL/6小鼠随机分为对照组、感染组(感染后2、4、6、8周组)。感染组小鼠用0.2 mL含400~500条旋毛虫的生理盐水灌胃。于感染后2、4、6、8周,记录小鼠体质量,检测腹壁撤退反射(AWR),连续8 h收集小鼠粪便,计算粪便含水百分数。HE染色观察肠道病理改变,免疫组织化学法及Western blotting检测回盲部和结肠中IL-17、occludin和ZO-1的表达。【结果】感染后第2周小鼠肠道出现急性炎症反应,体质量显著降低(P=0.000);至第8周肠道炎症和小鼠体质量基本恢复至正常。结直肠扩张容量为0.35和0.5 mL时,感染组AWR评分均高于对照组(P<0.01)。感染组粪便含水百分数均高于对照组(P<0.05)。免疫组化及Western blotting结果显示:与对照组相比,感染后2周组IL-17降低(P<0.05),感染后8周组IL-17增高(P<0.05);感染组occludin和ZO-1均低于对照组(P<0.05)。【结论】IL-17的动态改变及紧密连接蛋白occludin和ZO-1的减少可能是导致PI-IBS小鼠内脏高敏感性和粪便含水百分数增高的原因之一,参与了PI-IBS的发生。
【Objective】To observe the dynamic changes of intestinal IL-17,occludin,and ZO-1 in mice with postinfectious irritable bowel syndrome（PI-IBS）.【Methods】Forty C57 BL/6 mice were randomly divided into 5 groups：control group and infection groups（2 weeks,4 weeks,6 weeks,and 8 weeks after trichinella infection）. Infection groups were given by gavaging of 400~500 Trichinella spiralis in 0.2 mL of normal saline. The body weight of mice were recorded at week 2,4,6,and 8 after infection. The visceral sensitivity of mice was measured by the abdominal withdrawal reflex（AWR）. The stool was collected continuously for 8 hours to calculate the percentage of fecal water content. Pathological changes of gut were observed by HE staining. The expressions of IL-17,occludin,and ZO-1 in ileocecus and colon were detected by immunohistochemistry and Western blotting.【Results】At week 2 after infection,the acute inflammation of the intestinal tract was observed and the body weight of mice were significantly decreased（P=0.000）. Until week 8 after infection,the intestinal inflammation and body weight of mice recovered to normal. When the colorectal dilatation capacity was 0.35 and 0.5 mL,the AWR scores in the infection groups were significantly higher than those in the controlgroup（P〈0.01）. The percentages of fecal water content in the infection groups were significantly higher than those in the control group（P〈0.05）. Compared with the control group,the expressions of IL-17 were significantly decreased in week 2 group（P〈0.05）and increased in week 8 group（P〈0.05）. The expressions of occludin and ZO-1 in the infection groups were significantly lower than those in the control group（P〈0.05）.【Conclusion】The dynamic changes of IL-17 and the decrease of Tight junction proteins may be one of the mechanisms of visceral hypersensitivity and increased percentages of fecal water content. They may be involved in the development of PI-IBS.
作者
彭云丽
代迎欢
何琴
周旭春
PENG Yun-li;DAI Ying-huan;HE Qin;ZHOU Xu-chun(Department of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016 China;The Chongqing Key LaboratmT of Translational Medicine in Major Metabolic Diseases, Chongqing 400016, China;The People＇s Hospital of Longchang, Longchang 642150, China)
出处
《中山大学学报（医学版）》
CAS
CSCD
北大核心
2018年第3期341-348,共8页
Journal of Sun Yat-Sen University:Medical Sciences
基金
重庆市卫计委医学科研课题(2016MSXM002)
作者简介
彭云丽，硕士研究生，E-mail：1398162854@qq．com；;周旭春，通信作者，副教授，研究方向：胃肠道功能紊乱性疾病，E-mail：chqxchzh@163．com
