摘要
微型真核浮游生物是海洋生物群落的重要组成部分,对维持海洋生态系统的稳定发挥着重要的作用.本研究对黄海水环境样本的标记基因ITS进行Illumina高通量测序,探讨了黄海微型真核浮游生物的多样性及群落结构特征,并分析了其与环境因子之间的关系.结果表明:(1)基于标记基因ITS的高通量测序技术可获得较丰富的真菌、浮游植物、浮游动物种类,其中真菌OTUs>浮游植物OTUs>浮游动物OTUs,真菌reads数>浮游动物reads数>浮游植物reads数.(2)不同水域微型浮游生物的物种丰度及多样性程度不同,Ace指数及Chao1指数表现为南黄海>北黄海>东海北部>冷水团,Simpson指数及Shannon-Wiener指数表现为东海北部>北黄海>南黄海>冷水团,冷水团水域的物种丰度及多样性最低.(3)随着温度的升高,真菌、浮游植物、浮游动物reads数、OTUs均呈现出上升的趋势;随着盐度的增加,真菌的reads数、OTUs升高,而浮游植物、浮游动物的reads数、OTUs下降,其中浮游植物的reads数、OTUs显著下降(P<0.05);在北纬30°~40°范围内,随着纬度的升高,真菌、浮游动物的Shannon-Wiener指数上升,而浮游植物Shannon-Wiener指数下降;与海岸线的距离越大,真菌、浮游植物、浮游动物Shannon-Wiener指数越低.本研究首次以ITS为扩增子高通量测序研究海洋微型真核浮游生物,丰富了微型真核浮游生物的认识,揭示了高通量测序技术在该海域研究的可行性,为其他海域微型真核浮游生物的研究提供了借鉴.
Eukaryotic micro-plankton plays a key role in the marine biotic community and in maintaining the stability of the marine ecosystem. In order to determine the diversity and characteristics of eukaryotic micro-plankton in the Yellow Sea,Illumina highthroughput sequencing,based on gene markers of ITS,was conducted. An analysis of the relationship between community structure and environmental factors was conducted as well. The results are summarized in the following.（1） Various of species of fungi,phytoplankton,and zooplankton were obtained by high-throughput sequencing technology; for OTUs,the order is fungi phytoplankton zooplankton,and for reads,the order is fungi zooplankton phytoplankton.（2） In different waters,species abundance and diversity differed from each other. The Ace index and Chao1 index demonstrated an ordering of southern Yellow Sea northern Yellow Sea northern East Sea Cold Water Mass; however,for the Simpson index and Shannon-Wiener index,the tendency followed the order of northern East Sea northern Yellow Sea southern Yellow Sea Cold Water Mass. Species abundance and diversity in the Cold Water Mass were lowest.（3） The reads and OTUs of fungi,phytoplankton,and zooplankton increased as temperature rose. The reads and OTUs of fungi increased as salinity increased,while there was an opposite trend for phytoplankton and zooplankton. Latitude was another significant factor. In the range of 30° to 40° north latitude,the Shannon-Wiener index for fungi and zooplankton indicated an increasing tendency with an increase in latitude,while the Shannon-Wiener index for phytoplankton showed an opposite trend. With a greater distance from the coast,the Shannon-Wiener index of fungi,phytoplankton and zooplankton was lower. This is the first study that amplifies the use ITS in the research of marine eukaryotic micro-plankton,giving an enriched understanding of eukaryotic micro-plankton. The feasibility of applying high-throughput sequencing technology in the sea is proven,and this will provide a reference for other researchers.
作者
张莉
林佳宁
张远
王书平
张晓娇
ZHANG Li1,2,3, LIN Jia-ning2,3, ZHANG Yuan2,3 , WANG Shu-ping2,3, ZHANG Xiao-jiao2,3,4(1. College of Environmental Sciences, Liaoning University, Shenyang 110036, China; 2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Researeh Aeademy of Environmental Sciences, Beijing 100012, China; 3. Laboratory of Riverine Eeological Conservation and Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; 4. College of Fisheries and Life Seienee, Dalian Ocean University, Dalian 116023, Chin)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2018年第5期2368-2379,共12页
Environmental Science
基金
国家自然科学基金项目(41571050)
作者简介
张莉（1993～），女，硕士研究生，主要研究方向为水生生物学，E-mail：zhangli_03_18@163．com;通信作者，E-mail：zhangyuan@craes.org．cn
