摘要
目的探讨分析临床微生物检验和细菌耐药性的临床应用。方法本次研究所选的细菌样本为我院2017年2月至2017年4月各科室血、脓、痰、尿样本,所得到的细菌样本均严格按照标准操作规程进行培养、鉴定和分离,合计得到菌株500例。对临床送检的样本按照常规检验方法对病原菌进行分离,同时参照美国临床实验室标准化委员会(NCCLS)制定的一系列标准与指南对细菌耐药性进行相关检验,革兰阴性菌和革兰阳性菌的实验板条均为符合国际标准的实验耗材。结果本次研究分离出共计500株致病菌株,其中革兰阳性球菌280株(56.00%),革兰阴性杆菌220株(44.00%)。在分离出的菌株中较为常见的凝固酶阴性葡萄球菌、铜绿假单胞菌、枸橼酸菌、克雷伯菌、变形杆菌、肠球菌、大肠埃希菌、不动杆菌。金黄色葡萄球菌对青霉素和氨苄青霉素的耐药性已经到达完全耐药,凝固酶阴性葡萄球菌对于青霉素和氨苄青霉素的耐药性也完全耐药,肠球菌对于头孢他定具有较高的耐药性,达到了88%,而变形杆菌则对氨苄青霉素具有较高的耐药性,铜绿假单胞菌耐药性最高的为唑啉头孢菌素。结论细菌耐药性情况已经越来越严重,微生物的检测和细菌耐药性测试对于抗菌药物的选用十分必要,要准确了解细菌耐药性的变化为临床抗菌药物的使用提供参考,有利于院内感染的控制。
Objective To explore and analyze clinical clinical application of microbiological test and bacterial resistance. Methods In the study, choose blood, pus, sputum, urine samples in departments of our hospital from February 2017 to April 2017 as bacterial samples, carry on cultivation, indentification and separation according to standard operating procedures, and obtain 500 strains. Separate pathogenic bacteria of clinical laboratory samples according to routine detection method, carry on bacterial resistance test referring to standards and guidelines of National Committee for clinical laboratory standards（NCCLS）, and experimental strips for gram-negative bacteria and gram-positive bacteria are all in accordance with international standard. Results In the study, 500 strains of pathogenic bacteria were separtated, including 280 strains of gram-positive cocci（56.00%）, 220 strains of gram-negative bacilli（44.00%）. Among them, common bacteria includes coagulase negative staphylococcus, pseudomonas aeruginosa, citrobacter diversus, klebsiella pneumoniae, proteus, enterococcus, escherichia coli and acinetobacter. And staphylococcus aureus has been completely resistant to penicillin and penbritin. Coagulase negative staphylococcus has also been completely resistant to penicillin and penbritin, enterococcus has high resistance to ceftazidime, reaching 88%, while proteus has high resistance to penbritin, pseudomonas aeruginosa has high resistance to cefazolin. Conclusion Bacterial resistance has become more and more serious, it is necessary to carry on microbial detection and bacterial resistance test for antimicrobial drugs choice, to understand changes of bacterial resistance accurately and provide reference for clinical application of antibiotics, which is conducive to control nosocomial infection.
作者
许红
XU Hong(Xinjiang Uygur Autonomous Region the First Charity Hospital, Urumqi, Xinjiang, 83001)
出处
《智慧健康》
2018年第6期19-20,69,共3页
Smart Healthcare
关键词
微生物检验
细菌耐药性检测
临床应用
Microbiological test
Bacterial resistance detection
Clinical application
