摘要
研究表明,来源于肠道内G-细菌细胞壁的内毒素是促成酒精性肝损伤的关键因素之一。益生菌已成功用于处理酒精引起的与消化道泄露和内毒素增加相关的动物肝损伤模型及患者,然而其机制还不明确。以Caco-2细胞为对象,体外研究表明,通过添加鼠李糖乳杆菌B10上清液,阻止了酒精引起的上皮单层功能紊乱。采用Si RNA干扰沉默Caco-2细胞HIF-1α/2α基因,B10上清液改善酒精引起的屏障功能紊乱作用不复存在。该结果表明:B10的积极作用与HIF有直接的关系,其作用可能是上清液中的成分通过调节缺氧诱导因子上调了肠道保护因子表达,修复酒精所致肠道屏障功能紊乱。
Gut-derived endotoxin is a critical factor in the development and progression of alcoholic liver disease（ALD）. Probiotics have been used to treat animal models of alcohol-induced liver injury associated with gut leakiness and endotoxemia as well as human ALD. However, the mechanism of their beneficial action are not well defined. In vitro studies using the Caco-2 cell culture model showed that the addition of B10 supernatant prevented alcohol-induced epithelial monolayer barrier dysfunction. Furthermore, gene silencing of HIF-1α/2α abolished the B10 effects, indicating that the protective effect of B10 is HIF-dependent. This study provides a mechanistic insight for utilization of probiotics for the treatment of ALD, and suggests a critical role for intestinal hypoxia and barrier dysfunction in the development of ALD.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第2期37-43,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
科技部国家重点研发计划项目(2017YFE0105400)
作者简介
王玉华，女，1972年出生，博士，教授E-mail：yuhua-ww@163．com
