摘要
目的：探讨藤黄醇提取物对HCT116细胞侵袭的影响及其内在的分子作用机制。方法：采用Transwell侵袭实验观察藤黄醇提取物对HCT116细胞的侵袭能力影响,实时荧光定量PCR法检测MMP-7和Cyclin D1的mRNA表达水平,Western Blot法检测MMP-7和Cyclin D1的蛋白表达水平,免疫荧光观察β-catenin在细胞内的定位变化。结果：随着藤黄醇提取物剂量的增加,其抑制HCT116细胞的侵袭作用逐渐增强,β-catenin在细胞核内分布逐渐下降,MMP-7和Cyclin D1的mRNA和蛋白表达水平逐渐下降。结论：藤黄醇提取物可能通过抑制异常活化的Wnt经典信号通路来抑制HCT116细胞的侵袭。
Objective： To explore the effect of ethanolic extract of Gamboge on the invasion ability of HCT116 colon cancer cell and underlying molecular mechanism. Methods： The invasion ability of HCT116 colon cancer cell was identified by cell invasion assay. The expressions of mRNA of MMP-7 and Cyclin D1 were examined by real-time fluorescence quantitative PCR analysis. The expressions of proteins were detected by Western Blot analysis. The localization ofβ-catenin was tested by immunofluorescence confocal microscopy. Result： With the dosage of Gamboge increased,the rate of inhibition of HCT116 colon cancer cell was gradually increased and the distribution of β-catenin in nucleus was decresed. Meanwhie,the ethanolic extract of gamboge down-regulated the mRNA and protein levels of MMP-7 and Cyclin D1. Conclusion： The ethanolic extract of Gamboge might inhibit the canonical Wnt signaling pathway to suppress the invasion ability of HCT116 colon cancer cells.
出处
《中华中医药学刊》
CAS
北大核心
2018年第3期581-585,I0014,I0015,共7页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81473565)
江苏省中医消化病临床医学研究中心项目(BL2014100)
江苏省“十二五”中医药重点学科基金项目(JS1301)
作者简介
李悠然（1988-），男，江苏南京人，中医师，博士，研究方向：结直肠外科疾病。;谷云飞（1960-），男，江苏南京人，主任中医师、教授，博士研究生导师，研究方向：结直肠外科疾病。E—mail：guyunfeil27@126．com
