摘要
目的探讨不同样本预富集方法对流式细胞术分选单核细胞的影响。方法采集15例志愿者外周血,分别采用红细胞裂解法、羟乙基淀粉离心沉淀法和Ficoll密度梯度离心法进行样本预富集处理。台盼蓝染色法检测细胞活力,流式细胞术对单核细胞进行绝对计数并对各亚群比例进行分析。结果 3种方法制备样本的细胞活力均达到95%以上;红细胞裂解法与未进行预富集样本的单核细胞绝对计数和分群无明显差别,羟乙基淀粉法细胞分群不明显,Ficoll密度梯度离心法获取的单核细胞数量明显低于未进行预富集样本。结论外周血单核细胞流式分选技术中,红细胞裂解法进行样本预富集是一种简单、经济、高效的方法。
Objective To investigate the effect of different pre-enrichment methods on flow cytometry cell sorting of monocyte.Methods Peripheral blood of 15 volunteers were pre-enriched by three methods：erythrocyte lysis,hydroxyethyl starch sedimentation,density gradient centrifugation using Ficoll.Cell viability was detected by Trypan blue staining.The amount and the proportion of monocytes were analyzed by flow cytometry.Results The cell viability of the samples prepared by the3 methods was above 95%.There was no significant difference between the absolute count and grouping of monocytes in erythrocytes lysis and without pre-enrichment samples.The cell subgroup was not obvious by hydroxyethyl starch sedimentation.The number of monocytes obtained by Ficoll density gradient centrifugation was significantly lower than that without pre-enrichment samples.Conclusion Erythrocyte lysis was a high efficient method for monocytes flow cytometry cell sorting.
作者
杨红艳
焉力方
王如刚
周欣
YANG Hong-yan;YAN Li-fang;WANG Ru-gang;ZHOU Xin(Beijing Research Center for Preventive Medicine ,Beijing 100020 ,China;the Si3th Detachment of Tianj in General Team of Chinese Armed Police Force, Tianj in 300402, China;Heart Hospital, Logistics University of the Chinese Armed Police Forces, Tianjin 300162, China)
出处
《现代检验医学杂志》
CAS
2018年第1期128-130,共3页
Journal of Modern Laboratory Medicine
基金
国家自然科学基金(81570335)
作者简介
杨红艳（1969-），女．硕士研究生，副主任医师，主要从事职业病基础和临床研究，Email：yanghy1101@sina．com
