摘要
为了探讨致病性大肠杆菌内HMWP2蛋白的生物学结构和功能,采用Red同源重组技术,对致病性E.coli的HPI irp2基因进行敲除,成功构建了致病性E.coli irp2基因缺失株,并利用测序结果对HMWP2蛋白进行了多重预测分析,以期为致病性大肠杆菌相关疾病的防治和发生机理研究提供理论基础。
In order to investigate the biological structure and function of HMWP2 protein in PEC,the irp2 gene was knocked down by Red homologous recombination technique and irp2 gene deletion strain was successfully constructed, then the structure and function of HMWP2 protein was predicted and analysised by irp2＂s sequence This study aims to provide a theoretical basis for prevention and pathogenesis of E.coli-related diseases
出处
《中国兽药杂志》
2018年第2期12-18,共7页
Chinese Journal of Veterinary Drug
基金
国家自然科学基金(31260594
31660704)
云南省高校科技创新团队支持计划资助(云教科[2011]14号)
云南生猪产业技术体系建设专项经费资助(云财农[2009]171号)
作者简介
刘超英，博士研究生，从事特种经济动物健康养殖方面研究。;高洪。E-mail：gaohongping@163．com
