摘要
目的:分析皮肤鳞状细胞癌中p16、Cyclin D1、pRb蛋白表达与临床病理特征的关系为临床诊断提供依据。方法:采集45例皮肤鳞状细胞癌标本及10例正常皮肤标本,采用免疫组化法测定其中的p16、Cyclin D1、pRb蛋白表达情况。结果:皮肤鳞状细胞癌的p16蛋白阳性表达率明显低于正常皮肤,Cyclin D1、pRb蛋白明显高于正常皮肤,差异有统计学意义(P<0.05)。Broders分级为Ⅲ~Ⅳ级、有淋巴结转移者p16蛋白阳性表达率明显低于Ⅰ~Ⅱ级者(P<0.05),Cyclin D1、pRb蛋白阳性表达明显高于Ⅰ~Ⅱ级、无淋巴结转移者(P<0.05)。直线相关分析显示,p16蛋白阳性表达与Cyclin D1、pRb蛋白呈负相关;cyclin D1蛋白阳性表达与pRb蛋白呈正相关。结论:皮肤鳞状细胞癌的发生和进展可能是p16蛋白、Cyclin D1、pRb蛋白异常表达的结果。故认为可通过对三种指标进行检测来评估皮肤鳞状细胞癌的恶性程度及预后。
Objective:To analyze the relationship between the expression of p16,Cyclin D1 and pRb protein and clinicopathological features in skin squamous cell carcinoma,to provide basis for clinical diagnosis.Methods:45 specimens of skin squamous cell carcinoma and 10 normal skin samples were collected.The expressions of p16,Cyclin D1 and pRb proteins were detected by immunohistochemistry.Results:The positive rate of p16 protein expression in skin squamous cell carcinoma was significantly lower than that in normal skin,and the CyclinD1 and pRb proteins were significantly higher than those in normal skin,the difference was statistically significant(P<0.05).The expression rate of p16 in the specimens with Broders gradeⅢ~Ⅳ grade,lymph node metastasis was significantly lower than that with gradeⅠ~Ⅱ,without lymph node metastasis(P<0.05),the Cyclin D1,pRb protein positive expression was significantly higher than that withⅠ~Ⅱ,without lymph node metastasis(P<0.05).Linear correlation analysis showed that the positive expression of p16 protein was negatively correlated with Cyclin D1 and pRb protein.The positive expression of Cyclin D1 protein was positively correlated with pRb protein.Conclusion:The occurrence and progression of skin squamous cell carcinoma may be the result of abnormal expression of p16,Cyclin D1 and pRb proteins.Therefore,we can evaluate the malignancy and prognosis of skin squamous cell carcinoma by detecting the three indexes.
出处
《医学理论与实践》
2018年第2期177-179,共3页
The Journal of Medical Theory and Practice
